We narrowed to 551 results for: NOL
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TypeBlog Post...necessary), there are opportunities to work for biotechnology companies and help other researchers use the...experience and scientific knowledge they require. Biotechnology companies vary to what extent they hire experts...
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Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his... -
Lab to Office Culture Shock
TypeBlog Post... my work. The meetings often are the work. Will Arnold, one of the newer members of our Scientist team...and for modeling in the picture above), and Will Arnold for their ideas and tips! Additional resources ... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post... and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett syndrome mutations...three neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program... -
To Codon Optimize or Not: That is the Question
TypeBlog Post... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014). ... heterologous protein expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post..., Methylophilus methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...mediates targeted genome editing in plants. Plant Biotechnology Journal, 22(10), 2950–2953. https://doi.org/...through de novo induction of meristems. Nature Biotechnology, 38(1), 84–89. https://doi.org/10.1038/s41587... -
CRISPR Activation: A Practical Guide
TypeBlog Post...discovery through the creation of innovative technologies. To date his group has develop several popular...genome editing and transcription modulation. Nat Biotechnol 33:139–142 . https://doi.org/10.1038/nbt.3149... -
The Challenges of Cell Culture
TypeBlog Post...tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post.... To indicate the sequence, people often use terminology like “C-terminal fusion,” but I find this very...artifacts. This is an important downside of the technology and should always be considered when interpreting... -
Five Popular Model Organisms, Part 2
TypeBlog Post... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post... vectors carrying parts of a larger gene. Nat Biotechnol 20:697–701 . https://doi.org/10.1038/nbt0702-...-splicing adeno-associated viral vectors. Nat Biotechnol 23:1435–1439 . https://doi.org/10.1038/nbt1153... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner... -
Hot Plasmids: Summer 2024
TypeBlog Post...ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038... -
Tips for Getting a Faculty Position
TypeBlog Post...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum...