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We narrowed to 1,020 results for: NTS;

Showing: 301 - 320 of 1020 results

  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...essential components of both pathways have been shown to be efficacious for increasing knock-in events (Yang...mutations and gene editing Targeted genome editing events fall into two general classes: knock-in and knock-out...frameshift mutation. The majority of cellular repair events will generate these types of mutations, making ...DNA repair mechanism in general. On average, it accounts for less than 10% of DNA repair, with this rate...considerations for designing donor DNA for successful HDR events. CRISPR cut site location Position your CRISPR...disrupting mutations in donor DNA   Promoting HDR events over other edits Like I said earlier, HDR is not... to exist as a repair template. To maximize HDR events, ensure cells are actively cycling through the ...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...cell to perform multiple recombination events, all linear fragments need to get into the same cell. For ...3), producing multiple fragments with specific homologous ends. These fragments are assembled in vivo ...multiple recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled...Figure 1). As this approach requires no special reagents or purification kits, it is low cost and can be...protocol:  Fragment generation - Any linear DNA fragments can be assembled in vivo as long as they have ...PCR is the primary method to produce the linear fragments, as desired modifications and homologous regions...positive colonies. Transformation - the linear fragments are transformed into E. coli and plasmid assembly...

  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...of the vector components are HIV-based? A7: No single plasmid contains all the components necessary to ...The components are divided as follows: Transfer vectors contain minimal cis-acting HIV components: LTRs...specific experiments being performed and you should understand the guidelines for using such reagents as outlined... produced by co-transfecting three essential ingredients: the lentiviral packaging vector, a transfer ...Second-generation vectors are likely sufficient for most experiments; however, the 3rd-generation packaging systems...the 3'LTR in the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target...LTRs, PPT, RRE, and psi packaging signal. Viral components typically total <1.5kb, which is rarely more ...

  4. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...NGCG 8  Tips for designing base editing experiments in plants Choose a base editing platform depending...engineering through adenine base editing in plants." Nature plants (2018): 1. PubMed PMID: 29867128. 11. Li...Plant base editor platforms and their molecular components. Adenine base editors for plant We developed... could be used to generate stable genome edited plants via Agrobacterium-mediated transformation. We also...). Type Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9...transient assay. For stable transformation, regenerated plants may be subjected to genotyping. If successful base... Yuan, et al. "Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A." Nature...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...result presents an opportunity for troubleshooting and optimization of the transfection reagents and process...There are many, many different types of experiments carried out by scientists every day. Although the... controls that can be used for plasmid-based experiments. What are control plasmids? Generally, control...interpretation of the results. Typically, plasmid-based experiments employ transfection, negative, positive, and ...control allows you to examine if the transfection reagents or the transfection process itself has any cytotoxic... on gene expression in your target cells. In experiments employing gene targeting or genome editing technologies...results are reproducible from properly controlled experiments. The observed phenotype associated with the independent...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...unique tropisms are now available. These include variants with broad CNS or PNS transduction, cell type ... control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, ... 2021) to evolve new systemic capsids for both rodents and non-human primates (NHPs). These engineered...fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...not been tested in rats. For more information on reagents and protocols, visit https://clover.caltech.edu...frequently been used for screening candidate regulatory elements such as enhancers and promoters for cell type-...Tabebordbar et al., 2021) method, selecting for variants that were effective in C57BL/6J mice, BALB/cJ ...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...selection easier, as well as highlight some recent advancements within this field. Fundamental principles of...enable highly specific visualization of cellular components, typically a protein of interest. Fluorescent...ligands Effects on protein function and cellular components: It's important to assess how dye-conjugation... function and its propensity to bind cellular components. Some dyes target specific organelles like mitochondria...may prove challenging to stain intracellular compartments in in vivo model systems, so extra care must..., highlighting its reliability for long-term experiments (Salari et al., 2019). Photostability, the ability...experiment types: Different types of fluorescent experiments, such as using fluorescently tagged ligands or...

  8. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ... Identify reagents you will need, any required training, and a priority list of experiments you hope to...that they may want to consider for future work placements. The chance to learn and collaborate all while...which you can apply to. These structured types of placements often have some form of built-in professional... ensure your expectations are reasonable, your reagents are onsite before you need them, and you are set... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...transportation, living expenses, and necessary reagents. Your primary lab may be willing to cover these...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...of your various blot reagents. There are two types of gradients. Protein gradients use dilutions of your...background. Created with biorender.com   Both gradients can be informative, so spend some time thinking...particular question before running one.  Good on gradients? Excellent! Now, let’s head west, intrepid reader...   Ladders If you need very accurate protein measurements, consider using an unstained protein ladder,...conjugated antibody. If you do not need such precise measurements, you can use a colorimetric ladder, visible ... after your transfer. If you are expecting low amounts of protein, make sure your reversible staining ...wet and semi-dry transfers, you can adjust the amounts of alcohol and SDS in your transfer buffers. To...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...chemicals (biotinylation reagents). There are a variety of biotinylation reagents available that differ ...and thus different reagents are useful for biotinylation in different microenvironments. There are also ...organisms for life. Biotin is only synthesized by plants, most bacteria and some fungi, thus mammals must...unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin is...protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes can be easily...immunohistochemistry and antibody labelling. In these experiments, biotin is added to a primary or secondary antibody... ideal for signal amplification during these experiments. During detection, a biotinylated reporter enzyme...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    ...sequencing large amounts of DNA quickly and accurately. We're sure to see many improvements in a variety ...and sequencing gels, as well as their modern equivalents and see what we can learn from their evolution...DNA identity, size, and abundance. If a scientist wants to know if an organism has a particular gene mutation...confirming the results of cloning or knockout experiments to forensics and clinical diagnostics. One example...individual without sickle cell anemia, two DNA fragments are observed because the DNA has the site where...investigate DNA sequence content by comparing the fragments created by different restriction enzyme digests...allows a researcher to properly orient specific fragments of a DNA sequence of interest to create a physical...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... methods. Across these methods, the following components are needed: γ-Retroviral transfer plasmid encoding...similar to lentiviral packaging methods. The three components described above (envelope, packaging, and transfer...because once in the host cell cytoplasm, the viral components are small enough to enter the nucleus through...slightly different isoforms of these packaging components. Therefore, lentiviruses may not be efficiently...plasmid backbone is meant to be descriptive of the components found within the backbone. For example: pLXSN...particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein containing Reverse...Reverse Transcriptase and Integrase components. Transfer cPPT Central polypurine tract; recognition...

  13. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... of graduate students who are passionate about communicating science.  ComSciCon's events have 3 main ...apply for our future events. In addition to this annual event, graduate students in different regional...comscicon.com/. All our events are offered free of charge to accepted students, and we pay for travel ...Communicating Science Conference series for graduate students. I believe that communication is the single most...main goals: To help graduate students in all STEM fields become excellent science communicators. To empower...empower graduate students to become ambassadors for their respective fields. To help attendees launch ...practice science communication to more graduate students.   Each June, graduate student leaders in science...

  14. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...color changing flower involves two basic components: the pigments (anthocyanins), and the plant circadian...associated intellectual property concerns – patents and licensing agreements. Developing a product without the ... the rewards are great if you take the plunge – plants are the foundation of life on earth, and opportunities...feeds, foods, and fibers. And because working with plants can be challenging, there are a lot of unexplored...the complicated and unique metabolic processes of plants are being investigated on limited funding and time...also identified and characterized mechanisms and components of the circadian rhythm in petunias. Because ...before us, it’s possible for us to combine these components to develop flowers that change color. Without...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...vacuolar) pH environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can...facilitate innovative cancer research, the Reference Reagents Group of the NCI RAS Initiative, led by Dominic...method, which allows for easy assembly of DNA fragments by utilizing regions of sequence homology (usually...and test constructs composed of complex genetic elements. To demonstrate the usefulness of GAMP, the Jacks...promoters expressing GFP, tetracycline response elements, and shRNAs many of which are available at Addgene...allows for easy and rapid targeting of complex elements to specific genomic sites. For instance, the authors... community. If you plan to use GMAP for your experiments, think about depositing your new plasmids with...

  16. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...technology itself is constantly improving. Cas9 variants have been modified for genome editing, activating...specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. The off-target...substitutions throughout the groove in 32 separate Cas9 mutants. When tested for their ability to cut the EMX1 ...embryonic kidney (HEK) cells, 11 of these initial mutants retained the on-target activity of wt Cas9 while... of these mutations and tested the combination mutants for their on-target and off-target activity at ...locations in HEK cells. Further testing of two of the mutants, SpCas9(K855A) and eSpCas9(1.1), followed by a ...double strand breaks (BLESS), revealed that these mutants do not cause off-target effects at unanticipated...

  17. Technical Design of a Western Blot

    Type
    Blog Post
    ... compatible with detergents like SDS, while BCA assays are not with denaturing agents like DTT or β-mercaptoethanol...other hand, they are not as sensitive to lower amounts of protein, and can require more antibody or loading... loading buffer will not contain any denaturing agents. However, you’ll still have to lyse your proteins...protein. Proteins located in membrane-bound subcompartments, like the mitochondria or nucleus, will likely...gradient gel, the decision is probably already made: gradients are extremely difficult to handcast, so unless...blots. If you need to preserve sample, check the ingredients before using, as some contain Tween-20, which...reaction rate and are compatible with antimicrobial agents. Chemiluminescence kits When activating your enzyme...

  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...structures or other local genomic elements (Hazan and Bester 2021). Other Cas variants Another option is CRISPR...ncRNA (Jiang et al. 2014).  Simple knock-out experiments are trickier when it comes to long non-coding... et al. 2018). However, removing large lncRNA fragments with multiple gRNAs poses the risk of disrupting...can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and downstream...or introduced, into the break sites. Knock-in experiments are important for visualizing ncRNA localization...long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription of...CRISPR/Cas9 system is not effective for your experiments, there are plenty of other CRISPR-based options...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    ...only will these mStayGold variants be valuable tools for your next experiments, they will also likely inspire...genome editing for primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate... Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues...stability. Here’s a quick overview of these new variants:   Figure 2: Inner mitochondrial membranes...The Miyawaki lab generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and...Addgene here!   Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications...gene delivery capsids previously identified in rodents have not been widely successful in primates. The...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...contact A variety of reagents can increase transduction efficiency. These reagents typically enhance the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain...ensuring viral production is to perform PCR against components of the viral vector using the harvested viral... you time, and allow you to standardize your experiments later. Stay tuned to the Addgene blog and be ... pages to get up-to-date tips for your virus experiments. References 1. Anthony, Simon J., et al. "A...
Showing: 301 - 320 of 1020 results