We narrowed to 417 results for: NUC

...through each part of the cell on their way to the nucleus where their gene cargoes can be expressed. The...

...surface, in the cytosol, in mitochondria, or in cell nuclei. The two photosensitizers had similar rates of ...

...use the serotonin receptor to study how single nucleotide polymorphisms present in the human population...

...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...

...intended parents is “rehoused” in a separate, enucleated zygote with healthy mitochondria, with the intent...

...it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent problem...

...repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions...

...PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in...

...Microinjection requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the...

...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have...

...activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this platform accurately...

...Sanger sequencing determines the precise order of nucleotides within a DNA molecule, in this case a plasmid...

...the following information for a Cas enzyme: Nuclease properties (CasID): Cis/trans activity, PAM requirement...

...proteins: they contain the matrix, capsid, and nucleocapsid viral components needed for viral packaging,...

...the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several...

...DSB is repaired by NHEJ which depending on the nucleotides inserted or deleted, can result in an in-frame...

...antibodies. Genetic drift refers to changes in the nucleic acid sequence of the antibody-encoding genes over...

... to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded DNA breaks, ...

... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...

...estradiol for hER or progesterone for hPR - leads to nuclear localization, binding to the appropriate promoter...