We narrowed to 564 results for: PAC;

...the many freely-available DNA-editing software packages to assemble your expected full plasmid sequences...when designing a sequencing primer, you need some space between your primer binding site and the first feature...

...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ...the calcium sensors described are also already packaged into viral vectors and ready to go. Pro tip: Vectors...

...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection....   Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.  ...

...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...

...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...

...both technically challenging and can negatively impact zygote viability. Nanoblades, however, avoid this...this pitfall since they are injected into the space surrounding the zygote, where they can then fuse with...

...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...

...first keynote presentation. He talked about the impact of the Human Genome project on its 25th anniversary... how the genome project has already had a major impact on undiagnosed and rare diseases; Before the Human...

...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...

...with picking universities?” and “How does COVID-19 impact all of this?” are all very valid questions, and...program will allow for you to have the time and space to delve into what matters to you. It can also be...

...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that...

...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

...a team to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed...better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already...

... of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested ...was short-lived as powerful molecular graphics packages were soon developed that allowed researchers to...

.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence.... L., Medema, R. H., & van Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand...

...selection marker to generate stable cell lines. Packaging and Envelope plasmids provide the necessary components...AAV transduction Only compatible with SaCas9 (packaging limit ~4.5kb). CRISPR elements are inserted into...