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  1. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...a team to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed...better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested ...was short-lived as powerful molecular graphics packages were soon developed that allowed researchers to...

  7. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  8. PhD Applications After COVID

    Type
    Blog Post
    ...with picking universities?” and “How does COVID-19 impact all of this?” are all very valid questions, and...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  9. Common Injection Routes in Mice

    Type
    Blog Post
    ...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...

  13. Antibody Guide

    Type
    Collection
    ...antibodies are collected, tested against the antigen, packaged, and sold. This is the most cost-efficient way...antibodies to upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation...

  15. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...decided to take a different approach. His own most impactful learning experiences happened in the lab, and ...analyze their recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...well-documented history of racial bias, which still impacts and influences current research. Understanding ... reflects European ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency...

  18. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...counterparts, they will also be easier to deliver in low-capacity vectors, such as adeno-associated viral (AAV) ...
Showing: 301 - 320 of 555 results