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We narrowed to 399 results for: POR C

Showing: 301 - 320 of 399 results

  1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...stimulation of dual virus-expressing CR neurons. (C) Duration of painful responses in the left and right...changes. In addition to supporting in-person conference attendance, the award now supports virtual conference...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...hydropersulfides modulate protein activity and have important roles during oxidative stress. To monitor this...

  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...manipulation. Common inbred strains include C57BL/6, Balb/c, and 129. Conversely, outbred strains were originally...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...their monovalent nature. They are stable up to 80°C and can refold correctly after denaturing.  Designed...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    ...doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...islands (regions of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to ... versions of this reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27...The K9 reporter uses the HP1 chromodomain to recognise lysine 9 methylation, while the K27 reporter uses... methylation in living cells. Essentially this reporter construct consists of one substrate (the histone-derived...-K27 histone methylation reporter) positions on histone H3, which are known to be involved in repression...methylation of these sites was controlled, the reporter consistently showed significantly different FRET...states. Thus, this system is a reliable tool in reporting methylation status of these sites. This new technology...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ... PubMed Central PMCID: PMC3622210. Schek N, Cooke C, Alwine JC. Definition of the upstream efficiency ...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...degradation, and is integral to the nuclear export and translation processes as well.   Figure 2... non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use...the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1 Gag expression ...

  8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which can be used to confirm...Professor at Boston College. National reports stress the importance of providing authentic research experiences... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...

  9. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits" are identified...post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional...strong evidence that a particular gene is actually important for a given phenotype. Each hit from the screen...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...//doi.org/10.1016/s0091-679x(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ... AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki...nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop...

  12. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which result in the internalization...delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane...and gRNA expression       Plasmid DNA may be incorporated into the genome     Off-target effects can ...through a process known as CRISPR-EZ (CRISPR RNP Electroporation of Zygotes) (Chen et al. 2016). For CRISPR ...CRISPR experiments that involve HDR, electroporation can be combined with cell-type specific reagents in a ...a technique known as nucleofection, which forms pores in the nuclear membrane, allowing for entry of a... account with these types of experiments. One important consideration is that the Cas9-gRNA RNP will be...

  13. Pooled Library Amplifications

    Type
    Blog Post
    ...media, reducing the incubation temperature (e.g., 30 °C), and limiting your growth time (e.g., 16-18 hours...individual libraries can vary significantly, it’s important that we have an amplification protocol developed...and the desired coverage for the library (How important is it that every single plasmid in your library...protocol. Created with BioRender.com.   The most important factor to consider is your desired library coverage...not millions, of plasmids, they are generally electroporated into electrocompetent cells to ensure the highest...chemical transformations is less than that of electroporation, but an advantage is that chemical transformations...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...the most common uses, GFP can be fused to the N- or C-terminus of a protein, which allows the scientist ...a fluorescent emission wavelength in the green portion of the visible spectrum (hence the name), which...maintaining fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a promoter of...a given cell type. This type of transcription reporting was among the earliest uses of GFP. Fluorescence...protein-protein interactions. In this case, two portions of EGFP are fused to the proteins of interest,...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    ... targeting mCherry reporter (mChe), with co-incubation with assist peptide (AP). C) Workflow for Cas-RNP-PAGE...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized by pre-existing antibodies...

  16. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber...pair to another base pair at a specific site. 2. Temporal, spatial, or conditional control of CRISPR activity... way to make those genome edits. But, just as important is a way to flip the switch and turn off CRISPR...The ability to turn off CRISPR activity is an important step towards CRISPR-based therapies and for more...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

  18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...

  19. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...4. Mittereder, Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration and bioactivity... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...
Showing: 301 - 320 of 399 results