We narrowed to 443 results for: RELA

1. Components of CRISPR/Cas9

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   Blog Post

   ...use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome and a small RNA (gRNA) ...

2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...

3. Sweating the Small Stuff: Details in the Lab

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   ...double-checking details, or just taking a moment to relax. During really stressful days, I would even take...

4. Antibodies 101: Buffers, Storage, and Conjugates

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   ...can be very helpful if you're trying to find a relatively rare protein in an experiment with many proteins...

5. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ...control processes. For the many other COVID-19 related plasmids in our collection, we’ve relied on Sanger...

6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ..., in principle, these experiments could find correlations between variants in a gene of poorly classified...

7. Top Requested Lentivirus and AAV of 2016

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   ...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...

8. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ... present, it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to...

9. Plasmids 101: Inducible Promoters

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   Blog Post

   ...cells and typically only in the same or closely related species from which they were derived. Similarly...

10. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    ...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is...

11. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ... workhorse adeno associated virus (AAV) shows relatively low retrograde transport efficiency in most serotypes...

12. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post

    ...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...

13. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

    ...their work on the shoulders of giants. The still relatively young movement of sharing open hardware has a...

14. How Addgene Creates Content

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    Blog Post

    ...researchers in creating and sharing knowledge, related to the types of materials we help distribute and...

15. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post

    ...certain to choose your recommenders based on your relationships to the person as well as their current sphere...

16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ... a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged...

17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...closely together on several projects and this is one related to gene expression. One goal of the ENCODE project...

18. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ...technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential...

19. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...

20. Antibodies 101: Secondary Antibodies

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    Blog Post

    ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...