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...well. Making the transition from the bench can be jarring for the unprepared, and unfortunately, there aren...who referred to her lab as her family three times during a 10 minute conversation. Leaving a tight-knit ... “I’m a scientist.”    What should you expect during the lab → office transition? I’ve already provided...sure, but it also eliminates that feeling of floundering one can have when starting in an academic lab...science,” and virtually every person you talked to during the day was also a scientist. But now you also ...upsetting, there are ways to get back to the bench during your off hours. DIY bio labs are cropping up in...organizations like Science from Scientists, which try to bring real scientists into schools to provide students...

...editing in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018)....et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase with Cas9 nickase (nCas9). ...to an adenine base editor. A massive protein engineering effort by Gaudelli et al. resulted in the successful...it with dCas9, and transformed it into E. coli harboring the defective CamR gene. Screening for surviving...colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA*) that satisfactorily.... Kang, Beum-Chang, et al. "Precision genome engineering through adenine base editing in plants." Nature...

...Transposon-Associated TNPB system. In Springer protocols handbooks/Springer protocols (pp. 245–260). https:/... genomic sites that can be targeted with TnpB, offering access to regions previously unreachable with ...infect cells, have emerged as promising tools for delivering these reagents in both plant and animal systems... enabling more precise and accessible genome engineering. More elaborate protocols are available in our...Additional Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 RNA Interference... Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids...

... Career Planning Resources. The key to ensuring success during graduate school and beyond is to live in...transferable skills while working in the lab and by offering advice on areas where you might need some help...on what I have found to be especially beneficial during my own graduate career – and in fact, some of the... a job fair! Similar to a conference, job fairs bring you face-to-face with working professionals, with...the ACS career fair, which are larger meetings offering resources for students seeking employment within...graduate schools will even pay for you to attend!), exploring a job fair, and utilizing your local career development...

...Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene headquarters every...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one... September 13, 2018 This massive scale reagent sharing led us to calculate how much time we have saved...would have saved 5 million hours through reagent sharing. How much is 5 million hours? A colossal 547 years...plasmids to the repository and for the scientific sharing community (check out the infographic below for ...the past month, we’ve enjoyed seeing scientists sharing their contributions on social media as well the...

...Ellis does research in synthetic biology and bioengineering including a recent effort to construct a synthetic...Synthetic Biology and Innovation - A shared space for bringing synthetic biology to the UK with foundational ... SynBio. Chapter 3: Synthetic biology as the engineering of biology at the level of DNA using modular,...in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic production...lectures synthetic biology for the Department of Bioengineering. He graduated from Oxford University and then...supervision of Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one...

...Addgene has done a really good job at figuring out how to operate during the pandemic, but it’s important ... addition of Cicalese to the Board of Directors brings human resources experience and insight to the now... the near future? Right now it’s a matter of maneuvering through the impact of COVID-19. I think Addgene...which raises questions like “How do you start to bring people back to on-site work?” and “How do you start...not working so that I’m available to spend time caring for my mother. For fun, I enjoy pilates, jewelry...

...other blog posts on sharing science: 10 Ways to Share Your Science More Scientific sharing posts! ...Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... a wide variety of programs, including fall and spring lecture series, science cafes, and Reddit AMAs.... sharing... Mary Gearing...

...you for inspiring us with your breakthrough research and your commitment to scientific sharing. In the... of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check out this...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...continue to improve our services and work to make sharing even easier. We can't wait to see what you put ...

...mapping to investigate DNA sequence content by comparing the fragments created by different restriction...breakthrough for determining the order of nucleotides in a string of DNA (7). Sanger sequencing uses modified nucleotides...become obsolete. For instance, the popular genome-engineering tool, CRISPR, may one day make techniques used...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...under $1,000 (10). The future of sequencing should bring efficient, low cost methods for sequencing large... as both time and research progress. These will bring faster, higher throughput, and potentially more ...Andrew Cho, and Ashok B. Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols...

... shift toward hiring and promotion practices that focus on reproducibility and ensuring the health of ... at Harvard University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid...of different types of replication in Nosek and Errington 2017 and Schmmidt 2009, but for our purposes we...experiment wrong. However, even simple procedures like pouring a plate or running a gel have intricacies that ...science Twitter is very active with many scientists sharing their unpublished data. There are blogs - both ...novelty and impact. Journals could also begin requiring reproducibility checklists that at least make ...

...attB-attP pairs on a register to record all permuted substrings of a set of chemicals. Specific examples of arrangements...arrangements that should be able to record all permuted substrings of up to seven chemical inputs are given in Table...50% GC content) so that individual parts and/or strings of parts may be PCR-ed out of their current registers...population to correctly record each possible permuted substring of the inputs, and that five hour inductions were...cells to correctly record each possible permuted substring of inputs (see Figure S9 of our paper (1)). We...al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed PMID: ...Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

...biology and brings 10 years of research experience with stem cells to Transposagen's cell engineering team. ... sequence, making it very useful for genetic engineering approaches. Most importantly for this technology...selection cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR...Craig, “piggyBac transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110,...Sleeping Beauty Transposase Read up on Genome Engineering with FLEx Vectors  Learn how Homology Directed... genome engineering...

...Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring DA and other...someone is awake, agitated, or asleep. Though DA firing patterns (or the frequency of its release) are ...brain slices Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics... in live mice Report modulation of DA signaling during learning and reward consumption in live mice Find...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...We’re big fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what... what better way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot...ranks of our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV ...simple steps you can take to input your data for sharing on the site. The entry form has 5 general question...questions and so that we can give you recognition for sharing your data. Materials: What virus and serotype did... have any feedback at all, we’d appreciate you sharing it through this form. In honor of Open Data Day...

...from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody resource, which...community-building. The transparency begins with sharing sequence information for the antibodies in our ...accompany our antibody collection.  Addgene is sharing our protocols for recombinant antibody production... antibodies for your research. Please consider sharing your experiences with antibodies from Addgene via...other. Together, we can transform the antibody sharing landscape as well.     References Andrews NP, ...a nonprofit that has been supporting hybridoma sharing within the community for decades. Educational Resources...

...fluorescent proteins were best imaged after tissue clearing with SeeDB2 (Ke et al., 2016, 2018).   How to...amount of tTA to achieve highest expression levels. Clearing large tissues - Fluorescent proteins are very ...with ScaleCUBIC1 (Susaki et al., 2014), before clearing with SeeDB2. For more information, visit our ...action, watch the video below depicting dendrite wiring of mitral cells.   Many thanks to our guest... of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed...26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-...

...vector into the culture medium during adeno-associated virus manufacturing." Human gene therapy21.10 (2010...Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed PMID: 16528756...pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed PMID: 18613082...and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326... Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase,...

...reagent-grade endangered Martian chicory root, but considering this is a needle in a haystack type of objective...In retrospect I used a way too high Cas9 MOI considering that A549 cells are relatively easy to transduce...could avoid potential off target artifacts by comparing the phenotypes of multiple gRNAs. Targeting different...lanes 0.3 % 3.0, Figure 5, lane 2) is faint, so comparing it to the undigested control (Figure 4, lane 0...straightforward, and is a testament to how far genome engineering has come. For comparison, in 2007 it took about...for Designing Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing... genome engineering...

... Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share...applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the dynamics...protein binds to calcium it changes conformation and brings the two fluorescent proteins together thereby increasing... blue-shifted fluorescent protein (donor) and measuring the resultant emission from the red-shifted fluorescent...release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light. To avoid overlap...is available through Addgene (Plasmid # 87127). Pairing NanoLuc® luciferase with fluorescent proteins has...in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, 5023-33. PubMed...