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We narrowed to 1,010 results for: RING;

Showing: 301 - 320 of 1010 results

  1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...significant activity on some mismatch-containing pairings. During Golden Gate assembly, ligation of mismatched... assemblies that enable biosynthetic pathway engineering and optimization. However, current best practices...redesign was guided by both advances in the re-engineering of the original BsaI-HF® Type IIS restriction...efficiency and identification of mismatch-prone pairings. Using these 4 bp overhang observations, accurate...and a Development Group Leader overseeing a team bringing new products to market, New England Biolabs, Inc...comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining." Nucleic acids research (2018)...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... first identified in the late 1960s during research at hot springs in Yellowstone National Park.  In addition...Using these reagents and a series of heating (denaturing) and cooling (annealing) steps Taq polymerase... common for dNTPs and other reagents to run low during the final cycles which would slow or stop PCR amplification... as real time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires...this fluorescent dye, the concentration of the DNA during the PCR reaction cycles is continuously detected... Variations Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help us understand...nucleotides 1-10 of the guide RNA, prearranged for base pairing with a complementary DNA strand. These 10 nucleotides...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...mismatches in the next 8-10 bases after perfect pairing in the seed sequence.   Figure 6: Electron...stabilizing the active conformation of the HNH domain, ensuring all binding partners are in place before cleavage...still being uncovered, and different techniques all bring their own advantages—and blind spots—to these questions...better understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with...

  4. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...you to our depositors for sharing your science with us! Corralling and Cornering the Goldilocks Antibody...editing and multiplex prime editing (requiring minimal payloads!) Uncovering Ancestral Bias in CRISPR Research...antibodies for use in mice and human brain IHC and sharing the data on the mAb3D atlas website.  The iGluSnFR3...Beroukhim labs to write a post on their paper uncovering ancestral bias in CRISPR guide library design...

  5. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene headquarters every...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one... September 13, 2018 This massive scale reagent sharing led us to calculate how much time we have saved...would have saved 5 million hours through reagent sharing. How much is 5 million hours? A colossal 547 years...plasmids to the repository and for the scientific sharing community (check out the infographic below for ...the past month, we’ve enjoyed seeing scientists sharing their contributions on social media as well the...

  6. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...Transposon-Associated TNPB system. In Springer protocols handbooks/Springer protocols (pp. 245–260). https:/... genomic sites that can be targeted with TnpB, offering access to regions previously unreachable with ...infect cells, have emerged as promising tools for delivering these reagents in both plant and animal systems... enabling more precise and accessible genome engineering. More elaborate protocols are available in our...Additional Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 RNA Interference... Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids...

  7. Lab to Office Culture Shock

    Type
    Blog Post
    ...well. Making the transition from the bench can be jarring for the unprepared, and unfortunately, there aren...who referred to her lab as her family three times during a 10 minute conversation. Leaving a tight-knit ... “I’m a scientist.”    What should you expect during the lab → office transition? I’ve already provided...sure, but it also eliminates that feeling of floundering one can have when starting in an academic lab...science,” and virtually every person you talked to during the day was also a scientist. But now you also ...upsetting, there are ways to get back to the bench during your off hours. DIY bio labs are cropping up in...organizations like Science from Scientists, which try to bring real scientists into schools to provide students...

  8. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...editing in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018)....et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase with Cas9 nickase (nCas9). ...to an adenine base editor. A massive protein engineering effort by Gaudelli et al. resulted in the successful...it with dCas9, and transformed it into E. coli harboring the defective CamR gene. Screening for surviving...colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA*) that satisfactorily.... Kang, Beum-Chang, et al. "Precision genome engineering through adenine base editing in plants." Nature...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...the host cell. Some of these envelope-receptor pairings are broad, allowing delivery into many cell types...allows these viruses to infect neurons. Besides altering the host tropism of your virus, there are many...reasons when you might pseudotype your virus:  Lowering cytotoxicity. Different envelope proteins have... up less of the virus and is less affected.  Altering sensitivity to serum. Different pseudotyped viruses...the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA. This allows ...v12050513 Cronin J, Zhang X-Y, Reiser J (2005) Altering the Tropism of Lentiviral Vectors through Pseudotyping...Quick Guide to All Things Lentivirus Get tips for titering your lentiviral preps Resources on Addgene.org...

  10. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...pEcCas/pEcgRNA system. This system works well for engineering single genome edits but the double strand breaks...Cas9, a temperature-sensitive replicon for self-curing, an arabinose inducible lambda-red gene, and an... E. coli B and W strains. The optimized plasmid curing method was much simpler and took only ~32 hours...sites edited. We optimized the strategy for plasmid curing, providing users with a complete editing process...interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used the MUCICAT system in two...compared to the industrial strain. In our metabolic engineering case study of N-acetylglucosamine biosynthesis... systems to choose from. Ready for metabolic engineering with improved cargo dedicated to gene (cluster...

  11. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... Career Planning Resources. The key to ensuring success during graduate school and beyond is to live in...transferable skills while working in the lab and by offering advice on areas where you might need some help...on what I have found to be especially beneficial during my own graduate career – and in fact, some of the... a job fair! Similar to a conference, job fairs bring you face-to-face with working professionals, with...the ACS career fair, which are larger meetings offering resources for students seeking employment within...graduate schools will even pay for you to attend!), exploring a job fair, and utilizing your local career development...

  12. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...Ellis does research in synthetic biology and bioengineering including a recent effort to construct a synthetic...Synthetic Biology and Innovation - A shared space for bringing synthetic biology to the UK with foundational ... SynBio. Chapter 3: Synthetic biology as the engineering of biology at the level of DNA using modular,...in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic production...lectures synthetic biology for the Department of Bioengineering. He graduated from Oxford University and then...supervision of Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one...

  13. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...Addgene has done a really good job at figuring out how to operate during the pandemic, but it’s important ... addition of Cicalese to the Board of Directors brings human resources experience and insight to the now... the near future? Right now it’s a matter of maneuvering through the impact of COVID-19. I think Addgene...which raises questions like “How do you start to bring people back to on-site work?” and “How do you start...not working so that I’m available to spend time caring for my mother. For fun, I enjoy pilates, jewelry...

  14. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...other blog posts on sharing science: 10 Ways to Share Your Science More Scientific sharing posts! ...Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate... a wide variety of programs, including fall and spring lecture series, science cafes, and Reddit AMAs.... sharing... Mary Gearing...

  15. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...you for inspiring us with your breakthrough research and your commitment to scientific sharing. In the... of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid sharing? Check out this...for mammalian gene silencing. TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger...continue to improve our services and work to make sharing even easier. We can't wait to see what you put ...

  16. Evolution of Lab Techniques

    Type
    Blog Post
    ...mapping to investigate DNA sequence content by comparing the fragments created by different restriction...breakthrough for determining the order of nucleotides in a string of DNA (7). Sanger sequencing uses modified nucleotides...become obsolete. For instance, the popular genome-engineering tool, CRISPR, may one day make techniques used...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...under $1,000 (10). The future of sequencing should bring efficient, low cost methods for sequencing large... as both time and research progress. These will bring faster, higher throughput, and potentially more ...Andrew Cho, and Ashok B. Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ... shift toward hiring and promotion practices that focus on reproducibility and ensuring the health of ... at Harvard University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid...of different types of replication in Nosek and Errington 2017 and Schmmidt 2009, but for our purposes we...experiment wrong. However, even simple procedures like pouring a plate or running a gel have intricacies that ...science Twitter is very active with many scientists sharing their unpublished data. There are blogs - both ...novelty and impact. Journals could also begin requiring reproducibility checklists that at least make ...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share...applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the dynamics...protein binds to calcium it changes conformation and brings the two fluorescent proteins together thereby increasing... blue-shifted fluorescent protein (donor) and measuring the resultant emission from the red-shifted fluorescent...release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light. To avoid overlap...is available through Addgene (Plasmid # 87127). Pairing NanoLuc® luciferase with fluorescent proteins has...in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, 5023-33. PubMed...

  19. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...attB-attP pairs on a register to record all permuted substrings of a set of chemicals. Specific examples of arrangements...arrangements that should be able to record all permuted substrings of up to seven chemical inputs are given in Table...50% GC content) so that individual parts and/or strings of parts may be PCR-ed out of their current registers...population to correctly record each possible permuted substring of the inputs, and that five hour inductions were...cells to correctly record each possible permuted substring of inputs (see Figure S9 of our paper (1)). We...al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed PMID: ...Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...biology and brings 10 years of research experience with stem cells to Transposagen's cell engineering team. ... sequence, making it very useful for genetic engineering approaches. Most importantly for this technology...selection cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR...Craig, “piggyBac transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110,...Sleeping Beauty Transposase Read up on Genome Engineering with FLEx Vectors  Learn how Homology Directed... genome engineering...
Showing: 301 - 320 of 1010 results