We narrowed to 583 results for: Ret;
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TypeCollection...Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. ...
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Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...%} Interfering in the future While RNAi has retreated a bit to the background, it remains a solid option... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post..., you’ll first need to learn a little bit of theoretical background on the different parameters that your... tumor sample You made it! So much about the theoretical background. Let’s dive into the praxis of gating... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ... -
Transferable Skills Guide: Time Management
TypeBlog Post...entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The second way...during my day according to my priorities, I have a pretty good idea about the specific things I’ll be doing... -
Tips for Getting a Faculty Position
TypeBlog Post...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE... -
Five Popular Model Organisms
TypeBlog Post... (Zuber et al., 2009). By injecting mice with retroviral vectors that harbored different mutations found...21415126. PubMed Central PMCID: PMC3082451. Pfeiffer, Barret D., et al. "Refinement of tools for targeted gene... -
Plasmids 101: Biotinylation
TypeBlog Post...papers and webpages are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked...Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...composition. So back to the drawing board. Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that... -
Antibody Production
TypeCollection... ensure that potency and specificity have been retained. If the target antigen is not endogenously-expressed... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make... -
Antibodies 101: Validation
TypeBlog Post...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample... -
Arf GTPase Family
TypeCollection...Mammalian (pEGFP-C1) GEF Fbxo8 (FBX8) 26269 319 Gateway Return to top The content for this page was generated ... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no.... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...entire plate was scanned for V5 tagging using an Operetta imaging system. Dewari et al, eLife 2018;7:e35069...follow when planning epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene...