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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 601 results for: Ret;

Showing: 301 - 320 of 601 results

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR...editors (calling them GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    .... Other techniques, like electron microscopy and FRET, can offer more information on these regions.   ...SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., 2022). ...

  5. Ras Pathway

    Type
    Collection
    ...Frederick National Laboratory for Cancer Research Return to top Ras Pathway - Gene List Click on a name ...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway Clone Collection 2.0...

  6. Lentivirus Plasmids

    Type
    Collection
    ... Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and useful types...MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral Transfer Plasmids The...article for more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...major burden on the patients, their families and caretakers, and the health system in general. The required...Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian.... elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based method to monitor structural transitions in...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in a single well.... Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. ...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With so many options...g3.112.005439  Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...20 bp in length and paste them into contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins.... PMID: 28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ... FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV. 2015...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ...has both high binding capacity and high protein retention. It comes in a variety of pore sizes and protein...with high binding capacity, though it has lower retention after binding and washes. It’s less sturdy and...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... rule of thumb, the more strictly you want to interpret your blot’s data, the more time you’ll want to...starting a western blot, think about how you’ll interpret the data and what level of accuracy you’ll need...

  14. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Editors carrying the delta-984–1090 ADAR2 truncation retain RNA editing capabilities but are small enough to...Mammalian ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...

  15. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...

  16. Fluorescent Protein Guide

    Type
    Collection
    ...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein...

  17. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

  18. AAV Viral Preps

    Type
    Collection
    ... serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde AAV To target projection neurons Caltech...

  20. Institute for Protein Innovation

    Type
    Collection
    ...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...antibodies targeting families of cell surface and secreted proteins (glypicans and integrins) and common ...
Showing: 301 - 320 of 601 results