We narrowed to 622 results for: SET

...Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...

...interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome...

...mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...

...submitting patent or grant applications; to informal settings like lab meetings, social media, coffee chats ...

...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of...

...for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

...ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product and recipient...recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance, a...

...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

...your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF...

...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...

...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

...“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved...

...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

...current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “...

...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing...

...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

...its surroundings and being shaped by them. Another set of highly influential microenvironment cells are ...

...deposit to Addgene. We are also lauded by another set of stakeholders - the tech transfer community. In...

...solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly...