We narrowed to 730 results for: SON;

...slightly if you know a bit more about the other person. If you have a poster or talk at the conference...there parts of your elevator pitch where the other person seemed confused, bored, or particularly excited...after. For one conference I went to, I made it a personal goal to ask one question each day (this may be...

...., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019).....4124.  Kattnig, D. R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel...

...recommend using a Tris-based lysis buffer and sonication.  The obtained virions from cells and supernatant....2020.120 Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...L. H., Xiao, R., Lock, M., Lin, J., Korn, M., & Wilson, J. M. (2010). Efficient serotype-dependent release...

...efficiency. Figure used with permission from the Mark Dawson lab Re-transformation of the SPLINTR Libraries ...recombined backbones that may replicate faster. For this reason, if your library has inserts of different sizes... Hannah Dotson...

...is very tricky to use and fussy to optimize. One reason could be the instability of the supposedly alien...many may find this inconvenient. Another possible reason for the trickiness of NgAgo is the inability of... not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar, photography) and often...

...next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting.” Trying...I asked some colleagues to share valuable life lessons they have learned during their scientific training... them in a blog post on Born to Science: Life Lessons from Scientific Training More and more voices ...

...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74... Ross A, J. Hecksher-Sorensen, R. Baldock,  D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk.... Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et al. (2013) Nature, 497, 332-337. Pubmed. R....

...gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify this comparison, capsids...PubMed PMID: 28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants...

...Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation...a protocol for antibiotic selection, check out Harrison et al., 2006. Tips: Don’t plate seeds too densely... 24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method...

...the statistical independence assumption of the Poisson process. This modification also eases the interpretation...orders.  Our model factors the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t)....contains an intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects...

...genes that apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when...6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh, H. J., Bazinet, A. ...10.1182/BLOOD-2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, ...

...qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning our titering...for the detection of low copy numbers. For this reason, it can be used in the detection of rare sequences...PMID: 24328707. PubMed Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium...

...vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism...examine the consequences of manipulating genes. One reason for the success of zebrafish as a model organism... 2016). One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA...

...Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity.... Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

...screening! This blog was written by Addgenie Hannah Dotson.  Resources and references References Genome-...protocols.  References McDade JR, Waxmonsky NC, Swanson LE, Fan M. Practical Considerations for Using Pooled...  Hannah Dotson...

...mammalian cell lines. However, in those same comparisons, the AbuGoot lab reported no detectable effect...and can be easily programmed for new genes. In comparison with other large insert techniques like HITI ... Rachel Leeson...

...meeting who felt isolated at a conference for any reason was a Singleton. Figure 1: The Binning Singletons...Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis, Janet Iwasa,...Dunivin, Jen Greenwich, Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg,...

... can arise, and has arisen, for many different reasons. Exposure to antibacterial drugs provides a selection...households by observing proper cleanliness and personal hygiene standards. Handwashing is an essential...spread of antibiotic resistant bacteria from one person to the next. For more information and tips on preventing...

...lack of documentation and high turn-over of lab personnel. If you are unsure of the background of the lines... such as serum, other cell lines, or infected personnel and can persist undetected; unlike infections ..., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma infection significantly alters...

... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...