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...Additionally, proper citation of these Addgene IDs in published work allows readers to quickly track down the ...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org...

...made to moderate and cross-check all data with published literature as soon as possible after submission... queries.  Looking for a bright, monomeric FP published in the last few years with an emission peak around...

...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ...

...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For...

...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Do not use a 0.2 μm filter, as this is likely to shear the envelope of your virus. o. Virus may be stored...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

...exposure. Read the reagent’s material safety data sheet (MSDS) to determine if this is best. Check that ...biohazardous. Check your lab’s material safety data sheets (MSDS) to understand how to properly dispose of...

...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered...

...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

...Scientists Using Social Media" tip sheet from Science Media Savvy This tip sheet provides advice for tweeting...

...why the results you've measured should change established theory. So, what can you do to ensure that your...available. But think of this as a jumpstart or a refresher for your skillset rather than a permanent fix....

...homologue) or extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes...Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683...

...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...

...self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment protein....expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...the basic assay remains widely used today.  The sheer abundance of these critical research tools is a ...

...across the globe. This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli... Alyssa Shepard...

...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance ...

...expanded organoids. This work, and many others, pushed the field forward to where it is today. Below we...because of its potential for allogenic “off-the-shelf” treatment. In short, instead of using the patient...

...involves preparation of the tissue sample, several washes, and temperature changes to visualize the expression..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...