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  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is exceptionally ...

  3. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening...

  6. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...why the results you've measured should change established theory. So, what can you do to ensure that your...available. But think of this as a jumpstart or a refresher for your skillset rather than a permanent fix....

  7. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...homologue) or extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes...Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683...

  9. RNA Extraction Without A Kit

    Type
    Blog Post
    ... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment protein....expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...

  12. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    ...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...the basic assay remains widely used today.  The sheer abundance of these critical research tools is a ...

  18. CRISPR in the Clinic

    Type
    Blog Post
    ...expanded organoids. This work, and many others, pushed the field forward to where it is today. Below we...because of its potential for allogenic “off-the-shelf” treatment. In short, instead of using the patient...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    ...across the globe. This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli... Alyssa Shepard...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance ...
Showing: 301 - 320 of 596 results