Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 604 results for: She

Showing: 301 - 320 of 604 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is exceptionally ...

  4. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening...

  5. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...-80°C. Subsequent freeze and thaw cycles reduce shelf life. To recover bacteria from your glycerol stock...it from thawing completely and will improve the shelf life. It is very important that you shake the glycerol...

  6. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...why the results you've measured should change established theory. So, what can you do to ensure that your...available. But think of this as a jumpstart or a refresher for your skillset rather than a permanent fix....

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...homologue) or extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes...Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ..., and VP3, which together form the outer capsid shell that protects the viral genome. Helper plasmid E4...

  12. RNA Extraction Without A Kit

    Type
    Blog Post
    ... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater...

  13. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment protein....expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

  15. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...

  16. Working with Nuclear Receptors

    Type
    Blog Post
    ...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...the basic assay remains widely used today.  The sheer abundance of these critical research tools is a ...

  17. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Do not use a 0.2 μm filter, as this is likely to shear the envelope of your virus. o. Virus may be stored...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

  18. Weighing Reagents Protocol

    Type
    Protocol
    ...exposure. Read the reagent’s material safety data sheet (MSDS) to determine if this is best. Check that ...biohazardous. Check your lab’s material safety data sheets (MSDS) to understand how to properly dispose of...

  19. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... is fast and can cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org...Expanded serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes...
Showing: 301 - 320 of 604 results