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We narrowed to 927 results for: TIM;

Showing: 301 - 320 of 927 results

  1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...targeted region, as shown below.  To identify optimal nickase designs, IDT scientists Mollie Schubert...and Yan next examined a situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest ...13 nt away from the target site rather than the optimal <10 nt distance. Employing two gRNA sites that ...’s best advice: Use a PAM-out configuration Optimize your spacing D10A: nick sites separated by 37...in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome editing...

  2. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...will influence the stability of your sample over time and determine which experiments you can effectively... solution is fundamental, and it will impact the time you will be able to store your sample without having...preserving the stability of your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates...stability during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing...better than water in these conditions.  The next time you do a plasmid prep, plan in detail before you...

  3. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...attempting to put together such a presentation many times, I could never actually figure out what skills were... mastery. Scientists need to be doers for a long time before they master the skills they need to be successful... learners so we are all likely spending a lot of time in doer mode even late in our careers.  Managers...collaborating effectively with other teams. While they sometimes dig in and do some of the work, they must be more...others take the reins. They work alone much of the time, depending on the organization they lead. Effective...

  4. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...factors in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be ...harder to manage when the measure of work is not just time spent at the desk, but it is worth it. This requires...Good relationships with clients and colleagues Sometimes this means you have to fire a good performer if...deadlines (there’s that need for flexibility, sometimes taken to an extreme by scientists) Are highly ...

  5. Advice for Choosing a Research Project

    Type
    Blog Post
    ...you spend a good chuck of your mental energy and time. The research project also serves as a stepping ...productivity and direction of the project? What is your timeline? A typical research project can take several years... your project will be fresh as well.” Don't underestimate the importance of choosing a solid research ...proposal before even touching a pipetteman. Take the time to generate new ideas and think about them critically...George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists to ...

  6. The Strength of Story Telling

    Type
    Blog Post
    ...advancement. Scientists do and should tell stories all the time. They provide accounts of their research struggles...interview question: Interviewer: “Tell me about a time you had a conflict at work.” Interviewee: “I did...for a paper we were publishing, but when it came time to add author details a different person in the ...great example of storytelling – you get context, time, cause, effect, and a solid conclusion. Advice for...possible — if you find yourself jumping backwards in time to explain something, rethink your structure. Develop...

  7. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...multiple timepoints during reprogramming. These library-specific identifiers act like a timestamp and help...captures the gene expression of cells at a single time point, making it difficult to investigate how early... 10-week period. Their expression decreased over time but they were not silenced.    Find CellTag libraries...tagged with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...

  8. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...grafting different parts together. By combining two optimized elements of an antibody probe, scientists can ...some limitations. GFP has long protein maturation times which limits its use for imaging a protein’s entire...them hard to amplify and image for long periods of time.  Are antibody based probes a solution? To address... localization of single HA-tagged molecules over time.  The Stasevich lab also proved that HA frankenbody... a developing zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold...

  9. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...respect the time your Mentor is giving by following up on plans. Make a commitment to put time and energy... Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea to take larger goals (e.g...and record milestones in reaching goals. The more time and thought you put into setting goals, the more... posse holds me accountable for attending and sometimes it is only the knowledge that they will be watching...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... system, each isotype is expressed at different times of the immune response and initiates different immune...occur, known as genetic drift, meaning that over time, the antibody you expect it to produce is not necessarily...antibodies are plasmid-based. Their sequences can be optimized for specificity and sensitivity, for example by...synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies are just as ...Enzyme-linked Immunosorbent Assay). Immunoprecipitation Sometimes, instead of just detecting the presence of protein....2017.01589 Ayyar BV, Arora S, Ravi SS (2017) Optimizing antibody expression: The nuts and bolts. Methods...

  11. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    ...saves you from having to create a new fly line every time you want to express YFG in a new tissue type (more... new fly with hedgehog under a new promoter each time a new hypothesis is tested. Controlling multiple...running with fly lines that are already available, sometimes you’ll have to make your own. In my next post,...process of generating your own fly lines. In the meantime, be sure to browse the publication references ...expression systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...adjust our estimates based on the journal that published the articles.  That is, we re-estimate the citations...plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited with... This is also true for other journals, so our estimates now take into account any differences in citations...deposited at Addgene still accrue on average 2 to 3 times as many citations as other papers in the same journals...

  13. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...costs, and time away from home. You can choose your level of involvement and how much time you want to... biggest differences: you don't have to build in time between sessions. “At big conferences, you could... upvoted question.”If the Q&A session ran out of time, the speaker could continue to answer questions ...waiting for a session to start. “There was not much time to network,” Zhang says. “The schedule was pretty...

  14. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... we hear about issues with reproducibility, time and time again, the problem comes down to inadequate ... the same result. So, then they end up investing time that they thought would be moving forward instead...lucky and manage to escape, but you’ve lost a lot of time. It doesn’t have to be this way. We can never completely...paper is the one that you need to spend the least time on—just put down a basic outline and refer back ...

  15. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ... misfolding. But measuring variants one at a time is time consuming and unscalable.  Melissa Chiasson,...several rounds of dose and drug adjustments. Unger ultimately hopes that medical professionals can genotype...it study bacterial population dynamics with the TIMER growth rate bioreporter. During protein maturation...simultaneously monitor cell cycle and growth rate using TIMER and an unstable fluorescent protein reporter under...

  16. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...the degradation of over 600 cellular components ultimately leading to cell fragmentation and death (Elmore...approximately 20 fold by directed evolution. They then optimized the length of both linkers to generate a reporter...and detected fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, ...embryo development. GFP fluorescence increased over time and showed a clear pattern in the developing forebrain...Failure for cells to die or cells dying at the wrong time and/or place can lead to an array of diseases and...

  17. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...in his free time, he is pursuing a plant engineering project at BosLab. Once he optimizes plant cell culture...training prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer ...experience. However, after a few months of dedicated time at BosLab, he became our bacterial transformation... we’ve developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast...

  18. How to Lead a Great Meeting

    Type
    Blog Post
    ...meetings, we cut the time down to an hour or less and are able to provide more timely feedback on each other...idea why you are there. Make the most of everyone’s time by considering the level of meeting you need. Project...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...assigned items to keep the project moving. Use time wisely Meetings are for talking, not reading: Send...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...are powerful tools to measure real-time cellular reactions to stimuli and as well as changes in cellular...analyte present in the system at a given point in time. For example, if ATP was the molecule of interest... sensors are fluorescence-based and have been optimized for a wide-range of uses to monitor the fundamental...systems, rather than transiently overexpressing at the time of use. Another great viral perk for delivery is...

  20. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...promoter driving multiple combinations of WT, codon optimized, untagged, cleavage sites (thrombin and TEV), ... coding genes, tags, truncations, and codon optimizations similar to those found in the bacterial expression...plasmids Finally a set of wild type and codon optimized, full length and truncated versions of SARS-CoV... scientists without performing the full suite of time consuming, costly, and laborious QC for all several...re glad to be able to do so during this critical time. Visit our COVID-19 Plasmids and Resources Page!...
Showing: 301 - 320 of 927 results