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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 747 results for: UTY

Showing: 301 - 320 of 747 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...tet-off turns off constitutively expressed proteins while tet-on turns on constitutively silenced proteins...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... plasmids and co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission...tested the base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse ...Niemann-Pick disease type C, directly demonstrating the therapeutic potential for the split base editors. Find the...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...participating in science and engineering. “Fix the Institutions” promotes gender equality in careers through... shows that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers... both sexes ensures that the diagnostics and therapeutic interventions you develop represent the whole...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... interest. Endogenous gene tagging is an ideal solution because it allows for tagging and visualization...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...plasmid backbone for delivery to the cells. As a precautionary measure, it’s also good to make a few single...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ...as a STAR answer: a setting, a conflict, and a resolution. Stories usually have a theme too, which is something...your morning coffee, if that’s part of your daily routine. Stay hydrated. You might have lunch on your schedule...

  8. 6 Tips for Grant Writing

    Type
    Blog Post
    ...of expertise, equipment, or excellence of the institution). Likewise, if you’re staying in the same lab... realization that, if we want people to keep contributing to science, it would be helpful for them to ...we forget that some of the terminology we use routinely isn’t common to non-scientists and may make your...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...differing from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing ...der Oost J, Backofen R, Koonin EV. An updated evolutionary classification of CRISPR-Cas systems. 5. Nat...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...focus on one such strategy--the FLEx switch--which utilizes recombination elements to turn off expression ...introduced, recombination can proceed either by first utilizing the loxP sites or the lox551 sites. Regardless.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ... area on the outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science... “we underestimate living things.” He further cautioned the distinction of “higher” and “lower” life forms...of years of common ancestry with worms before evolutionary divergence. Nikolaus Rajewsky from the Max Delbruck...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...and to become involved in developing tools and solutions to research problems. This movement calls itself...Minnesota has declared our makerspace an educational institution meaning that we can obtain known mutants at $7.00... school to merge his interests in biology and computing. In 2009, he founded a team at UVic to compete...

  13. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can...Furthermore, among those Cas9 orthologs that have been scrutinized, the majority have been shown to exhibit more...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...DNA constructs designed for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust...when a protein is expressed through the use of constitutive and inducible promoters. Inducible promoters...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...

  15. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...the use of AdV for in vivo CRISPR-Cas delivery utilized the Non Homologous End Joining (NHEJ) DNA repair..., 2014). An alternative approach to NHEJ is to utilize the Homology Directed Repair (HDR) to knock in ...and very efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. ...

  16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...hard to make out the details. To increase the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR...enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab to simultaneously characterize...chromatin's dynamic role in gene regulation The high resolution and multiplexed chromatin ‘photos’ generated ...

  17. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...polyethylene glycol (PEG). We add 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration...need to determine the titer of your viral vector solution. A variety of titering methods have been developed...Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and...

  18. PhD Applications After COVID

    Type
    Blog Post
    ...department during the application process. Many institutions recommend that you reach out to potential advisors...you can’t visit. By talking with people at the institution, you can get a better sense of the environment...answer process can also help you to see if the institution is right for you outside of just the program ...

  19. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population... resistance to drugs and can identify future therapeutic targets for diseases causing uncontrolled cell...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to engineer two new IV ...stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant AAV capsid...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...
Showing: 301 - 320 of 747 results