We narrowed to 577 results for: Vit

1. RANbodies: Reporter Nanobody Fusions

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   ...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

2. dTAG - You're it!

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   ...depleting a protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing...performance, great depth of degradation, absolute selectivity, broad utility for degrading targets in different...

3. Transferable Skills Guide: Problem Solving

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   ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all... and taking on independent projects, you will inevitably develop problem solving skills passively. But...

4. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...necessary and sufficient for certain patterning activities of entire tissue types or even organs. In frogs...activation. Upon activation, exhibits stabilized activity for over 30 minutes and can be deactivated using...

5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications in which...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...

6. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...achieve maximal on-target and minimal off-target activities have proven themselves incredibly useful for ...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

7. Progress Towards a PAM-Free CRISPR

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   ...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep...

8. Hot Plasmids - October 2020

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   ...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

9. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...Another option uses optogentics to control neuronal activity, commonly via light-activated ion channels. Similarly...vectors By modulating neuronal gene expression and activity, you can use viral vectors to study all sorts ...

10. Hot Plasmids and Viral Preps - March 2021

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    ...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's...

11. Viral Vectors 101: Chemogenetics

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    ...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively...

12. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...ABE8s showed a median increase of 1.94x editing activity compared to ABE7.10. Depending on the position...evolution of adenine base editors with increased activity and therapeutic application. Nature Biotechnology...

13. Science communication: 9 Strategies to Get Your Foot in the Door

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    ... scared to engage with them. Comment on their activities and participate in discussions. Get your voice...Twitter to promote your other science communication activities, e.g. tweet it when you publish a new blog post...

14. Plasmids 101: Terminators and PolyA signals

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    ...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...

15. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...where each Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters...spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee within...

16. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ... and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed...Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be modified to assay...

17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ... proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection of synthetic...selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each mRNA molecule...

18. Of Myc and Men

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    ...of interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at...CRISPR experiments. It has certainly boosted our productivity in the Biohub. We hope it will do the same for...

20. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards ...cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all three germ layers, form ...