We narrowed to 581 results for: Vit

1. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ... of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing...

2. Transferable Skills Guide: Problem Solving

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   ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all... and taking on independent projects, you will inevitably develop problem solving skills passively. But...

3. dTAG - You're it!

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   ...depleting a protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing...performance, great depth of degradation, absolute selectivity, broad utility for degrading targets in different...

4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications in which...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...

5. RANbodies: Reporter Nanobody Fusions

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   ...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...necessary and sufficient for certain patterning activities of entire tissue types or even organs. In frogs...activation. Upon activation, exhibits stabilized activity for over 30 minutes and can be deactivated using...

7. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...achieve maximal on-target and minimal off-target activities have proven themselves incredibly useful for ...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

8. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ...ABE8s showed a median increase of 1.94x editing activity compared to ABE7.10. Depending on the position...evolution of adenine base editors with increased activity and therapeutic application. Nature Biotechnology...

9. Hot Plasmids and Viral Preps - March 2021

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   ...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's...

10. Science communication: 9 Strategies to Get Your Foot in the Door

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    ... scared to engage with them. Comment on their activities and participate in discussions. Get your voice...Twitter to promote your other science communication activities, e.g. tweet it when you publish a new blog post...

11. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...Another option uses optogentics to control neuronal activity, commonly via light-activated ion channels. Similarly...vectors By modulating neuronal gene expression and activity, you can use viral vectors to study all sorts ...

12. Hot Plasmids - October 2020

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    ...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

13. Progress Towards a PAM-Free CRISPR

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    ...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep...

14. Viral Vectors 101: Chemogenetics

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    ...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively...

15. Plasmids 101: Terminators and PolyA signals

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    ...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...

16. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...where each Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters...spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee within...

17. Hot Plasmids: Summer 2025

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    ... Addgene has tested these capsid plasmids in vitro and uses them in our own AAV production processes...for neuronal tracing. Labeling was nontoxic and activity-dependent, with more labeling across active synapses...

18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ... proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection of synthetic...selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each mRNA molecule...

19. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...excess energy at wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths...non-specific binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination...

20. Hot Plasmids and Viral Preps - May 2021

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    ...bead assay (Figure 2). After finding no cross-reactivity, antibody candidates underwent affinity screening... that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library...