We narrowed to 573 results for: Vit

1. Hot Plasmids and Viral Preps - March 2021

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   ...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's...

2. Hot Plasmids - October 2020

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   ...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ...ABE8s showed a median increase of 1.94x editing activity compared to ABE7.10. Depending on the position...evolution of adenine base editors with increased activity and therapeutic application. Nature Biotechnology...

4. Progress Towards a PAM-Free CRISPR

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   ...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep...

5. Viral Vectors 101: Chemogenetics

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   ...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively...

6. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...Another option uses optogentics to control neuronal activity, commonly via light-activated ion channels. Similarly...vectors By modulating neuronal gene expression and activity, you can use viral vectors to study all sorts ...

7. Science communication: 9 Strategies to Get Your Foot in the Door

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   ... scared to engage with them. Comment on their activities and participate in discussions. Get your voice...Twitter to promote your other science communication activities, e.g. tweet it when you publish a new blog post...

8. Plasmids 101: Terminators and PolyA signals

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   ...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...

9. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...where each Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters...spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee within...

10. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...bead assay (Figure 2). After finding no cross-reactivity, antibody candidates underwent affinity screening... that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library...

11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ... proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection of synthetic...selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each mRNA molecule...

12. Of Myc and Men

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    ...of interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some...

13. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards ...cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all three germ layers, form ...

14. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    ...Golden Gate assembly involves the simultaneous activities of a Type IIS restriction enzyme such as BsaI...base pair substrates, it demonstrates significant activity on some mismatch-containing pairings. During Golden...

15. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take a look at...CRISPR experiments. It has certainly boosted our productivity in the Biohub. We hope it will do the same for...

16. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ... and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed...Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be modified to assay...

17. Hot Plasmids: Summer 2025

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    ... Addgene has tested these capsid plasmids in vitro and uses them in our own AAV production processes...for neuronal tracing. Labeling was nontoxic and activity-dependent, with more labeling across active synapses...

18. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...excess energy at wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths...non-specific binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination...

19. Summer SciComm Series: Modes of Communication

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    Blog Post

    ...more challenging to design a hands-on interactive activity using elephants than it is to design one demonstrating...or do you need a full exhibit? In a five minute activity at a street fair, or a semester-long after school...

20. Antibodies 101: Producing Recombinant Antibodies

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    ...conformational changes in the antigen or lower detection sensitivity of the assay. Where possible, it can be useful...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....