We narrowed to 987 results for: abo

...occurring regions in the 5’ UTR of mRNA that bind metabolites, leading to conformational changes that control...Spinach-based riboswitches can be used as stable metabolite sensors for imaging in live cells. By placing...placing Spinach downstream of a riboswitch, metabolite binding to the riboswitch instead activates Spinach ...Dimerization of Broccoli increases its fluorescence by about 1.8-fold, and may be a better choice for imaging...modification that reduces nonspecific binding with RNA by about 7x). Mango fluoresces nearly as brightly as Spinach...Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate other fluorescent...

... Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies...Köhler who developed hybridoma technology. But what about single chain fragment variables (scFvs)? scFvs weren...from inclusion bodies, it’s time-consuming and laborious.  To avoid this, one or more of these approaches...antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...Hybridoma Bank Learn more about the SunTag system which uses an scFv to amplify the fluorescence intensity ...

...thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose metabolism pathway...little prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid... Phage resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...

...Addgene Blog Learn about Plasmid Awards to Researchers Working on Rare Diseases Read About Some of Our Outreach...Allied Sciences in Ghana. Read on to learn more about doctors Bengyella and Duedu and how they plan to...He hopes to teach his molecular biology students about the functions of these powerful tools and to use...China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International Shipping...

...models, showing that this transfer increases the metabolic and metastatic capacity of cancer cells. This ...macromolecules to serve cellular functions. Along with collaborators, the Denes Hnisz Lab previously discovered that...models By Brian O'Neill The Nadav Ahituv Lab and collaborators have found a way to make adipocytes fight cancer...of fat tissue that is able to waste energy by metabolizing glucose to dissipate heat. Of the genes they..., its accessibility outside of well-equipped laboratories remains limited. Members of the Stanley Qi Lab...production, allowing students to explore cell metabolism. Plasmid components of CRISPRkit are now available...

... on CRISPR Mouse Models Learn about Homology Directed Repair Learn about Cas9 Ribonucleoprotein (RNP) ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively...process of conventional gene targeting is lowered to about 2-3 months. But certain models remain more difficult... a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months. With CRISPR (B), you...directly into a zygote and obtain a knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded...

...USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories to start working...where individuals can exchange ideas, network, collaborate, and innovate internationally. Why an International...needs, and came from the universities mentioned above. The initial IMP cohort of 78 mentees included: ...    Other Resources Visit the IMP website Learn about the Mentor Circle Program at Brigham and Women's...Networking for Scientists Series Read our Blog Post about Choosing a Mentor      ...

...Addgene blog. In fact, we have so many blog posts about careers that we’ve compiled our top career blog ...wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive Director...school, I came across Joseph Campbell, and read about his "follow your bliss' mantra and never forgot ...Usually when I see inspiring artists who are thinking about getting into the arts (or any career to be honest...follow your passion. Do what you love. Don't worry about the rest.”- Jason Snair, Product Designer On the...

...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...

...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...particularly interesting. Although humans have known about bioluminescent organisms and their characteristic...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...for you, so if you’re interested in learning more about fluorescent proteins, I invite you to download our...

...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, ...for rerouting waste from the landfill, but what about all the manufacturing steps that happen before you...ACT label, which is an eco-nutrition label for laboratory products that emphasizes Accountability, Consistency...away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the...

...attracting so much attention. When I spoke with Daniel about the interplay of these two ideas he said “science... scientists sometimes forget how to communicate about their work with non-academic people.” Daniel went...perception, which is ironically what science is about. For me it is essential to stay in touch with the...unique samples, meaning that we know very little about these specimens - especially those which are now...Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on...

...a summary snapshot without telling you anything about outliers or data spread within those data sets. ...the rules” and which we have certain information about. The assumptions and requirements that need to be...Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way to go, which...only performing the comparisons you really care about. Family-wise error is the probability of a false...rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...not meet the assumptions for the tests described above (normal distribution, equal variance, and independent...

...Addgene blog Learn about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...Applications of transposons Now that you’ve learned about some of the popular transposon systems, let’s take...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...Beauty DNA transposon Learn about the piggyBac DNA transposon ...

...new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is composed... lentiviral expression (MuLE) system from the laboratory of Ian Frew simplifies the process of creating... seem preposterous. Luckily, the Pierre Neveu laboratory designed a new iterative, chaining-based cloning... to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried out five rounds...improved light-switchable bacterial TCSs Jeffrey Tabor's lab has developed new synthetic biology tools by...for controlling gene expression. For more details about these light-switchable TCSs, read our blog post:...

... of Bioinformatics reached out to us to tell us about a resource called Cellosaurus, a growing cell line...all the information in the resource.  This is a labor-intensive project; scripts can be used in some cases...preferences to help you identify proximal lines you care about. If you have cell lines without a certified origin... Or finding errors in the available information about a cell line you’ve used? Submit your manuscript ...distributor. A success all around! If you’re excited about this resource and want to learn more, check out ...

... for bag check was definitely worth it! Hearing about his passion for global health and the various initiatives...with fluorescent microbes. Check out her animation about the Hawaiian bobtail squid!  Continuing the art...talked to some scientists who stopped by the booth about amazing work already represented in the Addgene ...opportunity for Addgene to let microbiologists know about our mission of open science and resource sharing...community, contact [email protected] to find out more about our free deposit and distribution service! Did you...

...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...CRISPR technology in just 2 years and excitement about how much more there is left to do with the characterization...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

...section-specific comments, I re-read what I wrote about the paper after my initial read, adjust my language...wish for The last thing that I wanted to mention about the peer-review, is that as a peer-reviewer, you...each peer-review, I try to mention what I liked about this paper and recognize the hard work that the .../visualization using R/Shiny. She is passionate about open science, BIG data analysis and sharing whatever...review article Resources on Addgene.org Learn about depositing plasmids pre-publication Find molecular...

...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State University...mWasabi plasmids here! One of the most notable things about these proteins is that they are monomeric fluorescent... fluorescent protein blog posts Find blog posts about choosing fluorescent proteins for your research ...the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...