We narrowed to 407 results for: abo.4

...excessive nuclease activity in tissues (Li et al., 2018). 4. Selection marker while engineering viruses Acr genes...Bondy-Denomy et al., 2018). For example, AcrIIA4 mentioned above indicates that it’s the fourth Acr protein discovered...Cas9 Variant for Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org...

... 2000 Sep;81(Pt 9):2147-53. Pubmed PMID 10950970. 4. Lafon M. Rabies virus receptors. J Neurovirol. 2005...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...

...mutagenesis in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ, ...capacity. For instance, with a packaging capacity of about 6 kb, the 1st generation of AdVs have already a ... CRISPR/Cas9 research! To find more information about the adenoviral delivery of CRISPRS/Cas9 using the...

... 520.7547 (2015): 278-280. PubMed PMID: 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "... cancer are yielding promising results in the laboratory, and Addgene is accelerating this research by...within that pathway that are of interest to their laboratory. A single mouse click on a gene of interest will...

...Characterization of Long Non-Coding RNAs. Non-Coding RNA. 2021;7(4):79. doi:10.3390/ncrna7040079 Horlbeck MA, Liu SJ,... to devise an experiment that can tell you more about its function.  Resources and references Resources...

...that they will switch to other newer topics every 4-8 weeks. These topics are typically cutting edge; ...company to collaborate whenever it faces an important challenge. The types of projects mentioned above are just...and why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare...often work with such teams to help make decisions about R&D investments in either new internal efforts, ..., or through acquisitions of or collaborations with other companies. Medical Affairs groups work on building...building awareness among physicians and scientists about clinical research, drugs, the diseases they can ...professionals running the marketing, commercialization, collaborative, and financial functions associated with the...

...available for conjugation to a specific label (see step 4 for more information). Purify the Nanobody With A ...Control Protein Activity Learn more about protein tags Read about E. coli strains for protein expression..., if not better, than antibodies for all of the above listed molecular techniques, but they can also be...protein purification methods. Read on to learn more about nanobodies and how their structure and function ...between a fluorescent tag and the target antigen to about 2 nm, which can lead to higher resolution for super-resolution...nanobodies. If you’re interested, you can learn more about how nanobodies are identified in this review. If...

...guy from the lab was such a master drywall hanger?  4. Utilize your office space too Balance point number...early employees spent this remaining time by elaborately pranking each other, dressing in costume, and...frequently ask the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all day long, whistling...megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring...company.        Additional Resources Read other posts about life at Addgene!Read more of our careers posts! ...

...the National Grants Management Association (NGMA). 4. Develop a professional development strategy  Build...on becoming grant writers. When we asked others about how they became grant professionals, mid- to late-career...late-career grant professionals have similar stories about a time when their passion for an organization or... logic models, established partnerships and collaborations, and commitments to and proof of compliance...grants. It is no surprise that the U.S. Bureau of Labor and Statistics shows that employment of grant writers... skills & passion  Skills such as research, collaboration across multiple disciplines, writing, project...development strategy builds on the steps listed above and formalizes your development. You may consider...

... and worked as a technical support specialist for 4 years. Read on for insights from my time in technical...disadvantages of this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career ...general experimental guidance, and resolve complaints about products. As you might expect, different companies...order to enhance the OD signals and then complain about high background. In many instances, variations ...protocol. And customers may tell all sorts of details about the application, just not the ones that matter. ...communication, and is particularly excited to hear about novel developments in the fields of gene and cellular...Academic v. Non-Academic Career Decisions Learn about Science Career Options Explore Tips for Technical...

...Cancer 2.4 (2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover...Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about the Dangers...Misidentified or cross-contaminated cells have been known about since 1966 when Stanley Gartler reported that most...there is a loss of reputation that can impede collaborations, especially with industry. Today we also have...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

...relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447.  Lee...our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive fluorescent...of improved response. Then, just when Andrew was about to suggest she deprioritize the project, Maria’s...with a strong commitment to exploration and collaboration. Andrew is a physicist with a background in ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...interested in working on these questions or collaborating, the York Lab is eager to hear from you. Find...

...mediated knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright...CRISPR Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to ... post on increasing HDR efficiency to read more about optimizing this distance. Homology arms and types...screening strategies for your experiment. Also learn about Addgene’s knock-in plasmids for reagents to help...

...the same sample, you could end up with a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/... droplet generator, you will have to be careful about transferring your droplets from the droplet generator...Additional resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts ...collection of viral vector related protocols Learn more about the Addgene viral service ...

... PMID 25417800. PubMed Central: PMCID PMC4282009. 4. Yosef, Ido, Miriam Manor, Ruth Kiro, and Udi Qimron...organizations are working to help educate the public about proper antibiotic usage. Although these steps are...laudable, they likely won’t solve the growing problem. Above all, we need new antimicrobials, ideally ones for...used to deliver any of the three systems discussed above, with the caveat that phages are very diverse, and...

...endotoxin-free plasmid purification kits.     Figure 4: Schematic of the barcode matching system and PCR-... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...generated from lentiviral preparations (described above) that are subject to a concentration step prior ...

...dissolved in DMSO which allows both biotin and biotin-4-fluorescein to be dissolved at a higher concentration... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's... a cell. The Goedhart lab, Gadella lab, and collaborators have an ongoing effort to improve genetically..., that are optimized for excitation wavelengths above 1,000 nm and enable two-color imaging with red fluorescence...

...beads are collected by centrifugation or a magnet. 4) Unbound proteins are washed away. 5) The target protein...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

...Nature Genetics 28, 92-95. PubMed PMID: 11326284. 4. Bennett J, 2017. Taking Stock of Retinal Gene Therapy...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

...PMID: 18355003. PubMed Central PMCID: PMC2802578. 4. Xue, Lin, et al. "Imaging and manipulating proteins... the frontiers of biological imaging.  *Worried about your budget? Email [email protected] Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on Your Fluorescent ...