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...PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing the PCR product...to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA...polynucleotide synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. ...

...through 3 major steps to select for optimal sybody candidates. Figure 2: Outline of the process for selection...display mRNA from the enriched pool of sybody candidates are then reverse transcribed into cDNA fragments...library consisting of phages that display the candidate sybodies in their coat protein to screen for binding...-tag and a His6-tag to the end of each sybody candidate. These tags are used in a final enrichment step... narrowed-down pool (usually 10-30) of sybody candidates are sequenced for identification. All unique ...Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-...

...Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ...collection in your experiments and published your data, please fill out this form and we’ll add your citation...the following day. Nothing like waking up to fresh data! These same plasmids are then added into our normal...Spike Proteins. In: Methods in Molecular Biology. Humana Press, pp 331–338 https://doi.org/10.1007/978-1...

...delivery.  Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given...plex with two barcodes per cell. The researchers validated their work with the “signalome,” a 12-plex mixed-subclone...