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  1. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...

  2. To Codon Optimize or Not: That is the Question

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    Blog Post
    ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ... heterologous protein expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

  4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

  6. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ..., Methylophilus methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...

  8. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...

  9. Career Insights: Technical Support Specialist

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    Blog Post
    ...necessary), there are opportunities to work for biotechnology companies and help other researchers use the...experience and scientific knowledge they require. Biotechnology companies vary to what extent they hire experts...

  10. CRISPR Activation: A Practical Guide

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    Blog Post
    ...discovery through the creation of innovative technologies. To date his group has develop several popular...genome editing and transcription modulation. Nat Biotechnol 33:139–142 . https://doi.org/10.1038/nbt.3149...

  11. The Challenges of Cell Culture

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    Blog Post
    ...tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC...

  12. Hot Plasmids: Summer 2024

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    Blog Post
    ...ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  14. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum...

  15. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  20. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org...
Showing: 301 - 320 of 546 results