We narrowed to 393 results for: prep

1. Antibodies 101: Selecting the Right Antibody

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   Blog Post

   ...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...

2. Human Germline Editing Using CRISPR

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   ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...

3. Addgene's Tips for Plasmid Quality Control

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   ... over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward and reverse...

4. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   ...subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues you will ...

5. Making CRISPR Plasmids Using Fragmid

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   ...always, we recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing...

6. Antibodies 101: Stripping and Reprobing Western Blots

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   ...that section of the blot unusable for reprobing. Preparing your western blot Run your gel and membrane transfer...

7. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...riboprobes are created, the WISH protocol involves preparation of the tissue sample, several washes, and temperature...

8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   .... cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein functions have ...

9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ...first discovered as a contaminant of adenovirus preparations. It is an icosahedral, non-enveloped virus that...

10. Viral Vectors 101: Calcium Sensors

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    ...to go. Pro tip: Vectors with ready-to-use AAV preparations are labeled with the green virus particle on...

11. Viral Vectors 101: Virus Safety

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    ...If administering virus to mice, part of your preparation should be doing your best to be well-rested and...

12. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...of the line. With these tips in mind, you’ll be prepared to run an ELISA in your lab!   References and...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

14. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically...

15. Engaging with science and society at pgEd

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    ...running these events will be important both in preparing you for future work in these areas, and in helping...

16. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    ... opportunities around you. You just need to be prepared to take on the challenges and be proactive and...

18. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ...for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are busy...

19. Pooled Library Amplifications

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    ...Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled...

20. Transferable Skills: Negotiation

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    ...process felt unnatural and intimidating. I felt unprepared. Mainly, I thought of negotiation as something...