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We narrowed to 630 results for: proc

Showing: 301 - 320 of 630 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...

  2. Common Injection Routes in Mice

    Type
    Blog Post
    ...delivery is also common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia...similar to its intratracheal counterpart, as both procedures target the lungs. The main differences are that...

  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...Gene targeting takes advantage of this natural process to replace a targeted genetic locus with homologous... locus of interest. To give you an idea of the process, we’ll walk through an experiment designed to knock...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than... carbohydrates that can inhibit the cell lysis process. To ensure that lysis is complete, use a reagent...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...genes or entire pathways in a particular disease process. While CRISPR is certainly not the first means ... screen using CRISPR libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Therefore, these can be used as “timers” for cellular processes following their activation. The four main FTs ...Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov ...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    .... During the propagation and/or transformation process, some bacteria will either never receive a copy...antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp only affects...

  8. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  9. DNA Purification Without a Kit

    Type
    Blog Post
    ...and without degradation during the extraction procedure. Additionally, purified DNA was free of agarose...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...one is needed. For these reasons, we are in the process of transitioning our titering method from qPCR ...information needed for your PCR experiment. The process to titrate AAV by ddPCR begins with diluting the...

  11. MXS Chaining

    Type
    Blog Post
    ...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...and protein localization, as well as cellular processes like gene expression and cell migration (Sladitschek...

  12. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements ...tendency to brightly label somata but not neuronal processes. In contrast, smFPs can label fine neuronal structures...

  13. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sample name. If there was a minor error during processing, the window shows a yellow checked circle. Typically...results. If there are no results or there was a processing error, you will see a red exclamation point in...

  14. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ..., studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic...undifferentiated cells at the end of the screening process. However, while the neuronal marker TUJ1 is useful...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    ...the following email from a friend: “I'm in the process of onboarding a new employee who's coming directly...is good. You can experience a real onboarding process. Unless you were really lucky with your academic...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...specific RNAs out of a pool of RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...import and export regulations, differing customs processes, and the distances the packages needed to travel...addgene.org. Currently, we’re evaluating our depositor process to identify and reduce barriers for scientists ...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...transcript by endogenous processing enzymes (Figure 1). To ensure this tRNA processing only occurs on the RNA...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...to immunoprecipitation and simply refers to the process of anchoring an antibody, often called the capture...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...

  20. How to Be an Excellent Trainee

    Type
    Blog Post
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...training, while others prefer to review their notes, process what they’ve just learned, and ask questions later...
Showing: 301 - 320 of 630 results