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Showing: 301 - 320 of 618 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    .... During the propagation and/or transformation process, some bacteria will either never receive a copy...antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp only affects...

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  3. MXS Chaining

    Type
    Blog Post
    ...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...and protein localization, as well as cellular processes like gene expression and cell migration (Sladitschek...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    ...and without degradation during the extraction procedure. Additionally, purified DNA was free of agarose...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...one is needed. For these reasons, we are in the process of transitioning our titering method from qPCR ...information needed for your PCR experiment. The process to titrate AAV by ddPCR begins with diluting the...

  6. How to Be an Excellent Trainee

    Type
    Blog Post
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...training, while others prefer to review their notes, process what they’ve just learned, and ask questions later...

  7. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...import and export regulations, differing customs processes, and the distances the packages needed to travel...addgene.org. Currently, we’re evaluating our depositor process to identify and reduce barriers for scientists ...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...transcript by endogenous processing enzymes (Figure 1). To ensure this tRNA processing only occurs on the RNA...

  9. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...using Gateway technology To better understand the process, we’ll walk through an example experiment where...directly into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...others for failing to find our work?  I study processes for diagnosing and resolving organizational performance...impact of their contribution. It offers a 5-step process and several examples for creating effective research...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...doses and timings of their drug treatments. The process of rising to such challenges involves using knowledge...up-to-date information about the industry, recruiting processes, and can connect you to a community of colleagues...

  13. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...sample name. If there was a minor error during processing, the window shows a yellow checked circle. Typically...results. If there are no results or there was a processing error, you will see a red exclamation point in...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...to immunoprecipitation and simply refers to the process of anchoring an antibody, often called the capture...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...

  16. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ..., studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic...undifferentiated cells at the end of the screening process. However, while the neuronal marker TUJ1 is useful...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    ...the following email from a friend: “I'm in the process of onboarding a new employee who's coming directly...is good. You can experience a real onboarding process. Unless you were really lucky with your academic...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements ...tendency to brightly label somata but not neuronal processes. In contrast, smFPs can label fine neuronal structures...

  20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...specific RNAs out of a pool of RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...
Showing: 301 - 320 of 618 results