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We narrowed to 535 results for: val

Showing: 301 - 320 of 535 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas...0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid vectors for...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... line across the peak will indicate the cut-off value for a positive signal (Figure 8). Note that the ...Huwait, E., Gari, M., & Al-Qahtani, M. (2018). Evaluation of in vitro chondrocytic differentiation: A stem...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http://wesharescience.com...audiences that may otherwise never learn from the valuable research we are doing. Browse the site, learn ...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    ...whacky cell lines”. This may certainly have been a valid concern for the discipline (or lack thereof) in ...colleague?”. The main difference is the QC and validation carried out by the commercial repository. Repositories...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...notebook starts with defining your lab’s needs The values of ELNs show in the long run as they enable researchers...part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities such...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...immune response. The second strategy is the simple removal of the HIV-1 genome from infected cells, an idea...approaches, and each of these gRNAs will need to be validated for high on-target binding and low off-target ...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...species, so be sure to check the literature and validate your choice of AAV vector for your experimental...administration doses. Don't forget to submit your validation data after your experiment! Thanks to Addgenies...

  13. A Control for All Seasons

    Type
    Blog Post
    ...verification approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only...resources on the Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   LexA/lexAop A second binary ...plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...doi.org/10.1534/genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step...doi.org/10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R ...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...screening or easily shuttle the optimized, expression validated GPCR sequences to any desired backbone.  Kroeze...26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi Arumugaswami lab at...convenient experimental use. The plasmids were validated by both Western blotting and immunohistochemistry...

  17. Important Considerations When Using AAVs

    Type
    Blog Post
    ...cells an AAV can infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, ...235 (2014): 331-348. PubMed PMID: 25107853. 12. Valdmanis, Paul, and Mark A. Kay. "Future of rAAV gene therapy...

  19. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...propagate your plasmids in: DH5α, NEB Stable, or ccdB Survival.­ Whenever possible, we propagate plasmids in ...such as Gateway vectors, we recommend the ccdB Survival strain. Figure 10: Enter the growth and distribution...

  20. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...significant A to non-G conversion at target loci. The removal of inosine from DNA is likely infrequent, thus ... Browse CRISPR plasmids for base editing Find validated gRNAs ...
Showing: 301 - 320 of 535 results