CRISPR 101: Non-Homologous End Joining
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Blog Post
... of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing...
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dTAG - You're it!
TypeBlog Post...depleting a protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing...performance, great depth of degradation, absolute selectivity, broad utility for degrading targets in different... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications in which...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...necessary and sufficient for certain patterning activities of entire tissue types or even organs. In frogs...activation. Upon activation, exhibits stabilized activity for over 30 minutes and can be deactivated using... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all... and taking on independent projects, you will inevitably develop problem solving skills passively. But... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...achieve maximal on-target and minimal off-target activities have proven themselves incredibly useful for ...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post... scared to engage with them. Comment on their activities and participate in discussions. Get your voice...Twitter to promote your other science communication activities, e.g. tweet it when you publish a new blog post... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...Another option uses optogentics to control neuronal activity, commonly via light-activated ion channels. Similarly...vectors By modulating neuronal gene expression and activity, you can use viral vectors to study all sorts ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's... -
Hot Plasmids - October 2020
TypeBlog Post...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...ABE8s showed a median increase of 1.94x editing activity compared to ABE7.10. Depending on the position...evolution of adenine base editors with increased activity and therapeutic application. Nature Biotechnology... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...where each Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters...spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee within... -
Hot Plasmids: Summer 2025
TypeBlog Post... Addgene has tested these capsid plasmids in vitro and uses them in our own AAV production processes...for neuronal tracing. Labeling was nontoxic and activity-dependent, with more labeling across active synapses... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards ...cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all three germ layers, form ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...Golden Gate assembly involves the simultaneous activities of a Type IIS restriction enzyme such as BsaI...base pair substrates, it demonstrates significant activity on some mismatch-containing pairings. During Golden... -
Of Myc and Men
TypeBlog Post...of interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some...