We narrowed to 911 results for: TRO

...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...33714753. Voltron2 voltage sensors By Brian O’Neill New viral vector preps expressing the Voltron2 voltage...performance.      Figure 4: Structure of Voltron or Voltron2 conjugated to a JaneliaFluor dye emitting... SARS-CoV-2 research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia...membrane protein (Plasmid #141386-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid...also immunoblotted against vinculin as a loading control (right panel). Image from Addgene. These antibodies..., are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure...

...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma Our 293T...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ... April 2016) also performed by ddPCR.  Quality Control Addgene ensures high quality viral vectors by optimizing...production protocols and performing rigorous quality control (QC). The specific QC experiments performed varies...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...

...and other proteins controlled by GAL4 and ZIF268. To demonstrate independent control of gene expression...a GAL4/estradiol controlled transcription factor and a ZIF268/progesterone controlled transcription factor...transcription factor 1 controls expression of transcription factor 2, which directly controls expression of a...TRIM21 recruits ubiquitin and the proteasome to destroy antibody-bound pathogens. Clift et al. found that...can deliver these components together using electroporation. In these bulk cell experiments, Clift et al...no stress or damage upon TRIM-Away, and the electroporated cells re-adhered to the culture plate within...chemically inducible approaches to provide temporal control, this new system, Fkbp/Frb Inducible Recruitment...

... rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological...Listen to this episode of Hot Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed ...protein domains, they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et...opens up the potential for spatial and temporal control in many genetic targeting and editing experiments...electrical activity in the brain by inserting electrodes into the brain. But this process is time consuming...photostable, and is compatible with optogenetic control. The researchers also showed that Archon1 is functional...cloning podcast segment Recently, the Grefen lab introduced the “2in1” vector cloning system - a Gateway ...

...described the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2),...scientists have developed many optogenetic tools to control cellular activity with exquisite precision using...light. Optogenetics, or light activated tools, can control everything from electrical activity to gene expression...BioRender.com.   Optogenetic tools Types of control We will discuss three types of tools: light-gated...hyperpolarize the cell (Mattis et al., 2012). Bicistronic vectors combine excitation and inhibition by ...Some tools turn on and off rapidly with light to control precise timing of the perturbation. Other tools...intra-cellular signaling cascades. Optical methods for controlling GPCR activation have been developed to selectively...

...efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses that can only infect...Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral packaging...Lentiviruses are part of the retroviral family. What scientists often refer to as “retroviruses” are technically ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses and ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are...and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), the isoforms of these...LTRs) are different. As a result, lentiviral and retroviral packaging vectors are not interchangeable. While...

...produced in high-titer retroviral stocks generated by the transfer of a retrovirus into the packaging cell...are interested in using lentiviral vectors to introduce your favourite gene into your favourite cell line...CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector background Until the development of viral...cells, labs use viral vectors and particularly retroviral vectors because they target a broader range of...into the genome is more efficient. The first retroviral vectors used were derived from the Moloney Murine...MoMLV, lentiviruses, a separate genus of the Retroviridae family including human immunodeficiency virus...infect non-dividing cells is not restricted to in vitro cell culture as lentivirus-derived vectors are capable...

...inefficiently our edits were being introduced. Although we were able to introduce the desired mutations in genes...The CRISPR/Cas9 com­plex is very efficient at introducing double stranded breaks (DSBs) into genomic DNA...generating gene knockouts, NHEJ repair does not allow introduction of specific sequence changes. To generate ...Accomplishing this usually involves simultaneously introducing a homologous DNA repair template, such as a sin­gle-stranded...in our lab. Our goal was to use the system to introduce disease-causing mutations in iPSCs (or correct... but had never been systematically analyzed: introducing silent CRISPR/Cas9 blocking mutations in either...target sequence once the desired edit has been introduced. We found that blocking mutations increased the...

...Callaway EM. Retrograde neuronal tracing with a deletion-mutant rabies virus. Retrograde neuronal tracing...a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools for visualizing...that cell, and nascent RABV cross synapses to retrogradely infect neurons that project to the starter cell...occurs, subsequent cycles of viral replication and retrograde transmission occur, spreading the virus through...RABV G protein is necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham...2007). Thus, RABV transsynaptic spread can be controlled by selectively supplying the G protein in trans...released from the starter cell and transmits retrogradely to first order neurons, via entry mediated by...

...AAV9 serotypes have strong neuronal tropism, AAV5 targets both neurons and astrocytes, and AAV4 targets ...receptor subunits or neurotrophic factors. Another option uses optogentics to control neuronal activity,..., behavioral neuroscientists can now precisely control where and when certain genes are expressed to investigate...specific behavior. This blog post will give an introduction to behavioral neuroscience, and address experimental... in a laboratory, a high level of experimental control is required, because seemingly tiny external factors...Experimenter Interaction should be minimal and strictly controlled; optional handling animals Experiment Behavioral...channels that are chemically activated. Both are introduced in neurons and depending on the choice of channel...