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...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...

...included in them for downstream control – this is important later! Next, additional components are added -...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

...stylistic consistency. If you’ve ever doubted the importance of copy editors, the New Yorker’s “Comma Queen...review were discussed. Science communication opportunities If you are interested in the science communications...summer program that gives graduate students an opportunity to work in radio, print journalism, and other...

... RNA templates to DNA but suffer from several important shortcomings including low processivity (ie. the...29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed by Marcy...functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit includes ...

...purified protein involves immobilization on a solid support. This allows washing away of unbound material to...binding kinetics of the target ligand. Another important consideration is whether the affinity of an experimental...acids. Unfortunately, the location of biotin incorporation and to a lesser extent, the number of added ...

...with every possible option, we place the most important controls front and center, and make specialized... are in the pipeline. First, now that SnapGene supports “Collections”, which are shareable databases of... missing from our database, or if you see an opportunity to harness common feature detection in a new ...

...(Addgene #104102 - #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110...Figure 3) that can be introduced using in utero electroporation. Then at your preferred time points, sacrifice...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...

...new tools and protocols that they can easily incorporate into their own labs. This week we are pleased...following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose or other...test this approach with different capsids. Most importantly, because our AAV yields are improved we will ...

...probes, such as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs...of antibody-combining sites within the variable portions of heavy and light chains. Proceedings of the ... PMID:4560694. PubMed Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin...

...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...

...sort of talent agency for scientists, where we support health science graduate students with innovative...fund that would apply a percentage of returns to support student projects, but it quickly became apparent...The composition of the management team is more important than the product itself -- investors would rather...

...said. “We know CREB is really important, but where is it really important and when? That is not known.”...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...for both research and commercial purposes. It’s important to note that it’s the depositing institute that...signatures from two different institutes. Our online portal automates a lot of the process, from notifications...

...scientists. In addition to maintaining a laboratory to support both individual and community science projects,...experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept... involvement and provide all our members the opportunity to do molecular biology, we’ve developed community...

...when performing amplifications, and we use electroporation to ensure a good transformation efficiency....library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

...samples stored in our AAV tubes (Fig. 1). It’s important to note that the change in pH only occurred when...., 2014). Based on our results and the results reported elsewhere, we recommend that once you receive ...MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat Methods. 2013 Apr;10...

...how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight...to all the optional sexual health sessions and reported the intel back to my shyer classmates. For my ...the community During my PhD, I’ve also found opportunities for more hands-on science communication. Between...

...physiologically-relevant systems, to catch all the important interactions. It’s also important to determine if the human interactors...includes a GFP construct as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI...

...-hyg will be used to create gene deletions of important unknown regulatory regions in Pseudomonas and ... into a bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

...difficult during the pandemic era, with fewer opportunities for spontaneous conversations. What we are missing... minds. The final screen can also be saved and exported as a picture. I believe that showing something...fades away the longer we are on it – so it is important to take breaks. After 1 hour (or another designated...

... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...children. When I had my baby, we became closer. She supported me and answered my questions and honestly, she...

... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

...γ-retroviruses differ from lentiviruses? There are a few important differences between engineered γ-retroviruses ... enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long...

...of posts explains how to critically assess the reported properties of fluorescent proteins, how to do ...detected signal and therefore high brightness is an important feature of a fluorophore. The key question is ...However, the theoretical brightness ignores several important experimental conditions that are related to the...

...histones and chromatin architecture to every diagram portraying some aspect of eukaryotic DNA would become busy...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...

... in sample treatment across methods can affect reported titer by either method so to ensure equal animal...AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction. While...marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and link for ...

...as well. In a screen, you aren’t killing off a portion of the cells like in a selection. Instead, all ... genome, and scientists have found that when a portion of the gene is deleted, it creates a non-functional...Complementing this mutation by expressing this deleted portion, for example on a plasmid, creates a functional...

.... The advantages of optogenetics lie in the spatiotemporal control offered by the system that allows for... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...

...producing long unspecific RNA fragments that are incorporated into the dsRNA necessary for RNAi. The Vellai...adjacent to the T7 promoters to prevent the incorporation of non-specific nucleotides into the dsRNA leading...enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific...

...is to understand the function of the non-coding portion of the genome. Mendenhall and Richard Myers of ...efficiency of inducing double strand breaks and more opportunity for homologous recombination. Before CRISPR, ...versions of this out there. Addgene: Why was it important to you to make these plasmids available to other...