We narrowed to 949 results for: app

...used Parkinson’s experimental model. In fact, approximately 25% of yeast genes have a human counterpart,...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms transcribed...single TF was delivered to each cell, future applications could deliver multiple TFs to each cell, allowing...and/or TF dysregulation. A targeted TF screening approach can not only lead to a better understanding of...

...perform the experiments to demonstrate that this approach was useful in humans. Then, one of them heard ...has been the realization that there is a great untapped potential to make science more efficient, open...activity is non-profit and pro bono. If this sounds appealing, join us and help make science a better place....

...virtually) will make the relationship stronger.  If appropriate, invite them to speak for your organization, ...doesn’t work out, it’s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who...with peers and people around you naturally. This approach is much more likely to pay off in the long run...

... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

...(Schrallhammer et al 2012). This made them an appealing target for bioengineering, but it wasn’t clear...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...

...flank the insert. You will need to know both the approximate size of the vector backbone as well as the predicted...you have loaded in each well will allow you to approximate the DNA mass of comparably intense samples of...the gel when the bromophenol blue dye line is approximately 75-80% of the way down the gel will ensure you...

...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...dream lab While none of us will feel completely happy at work all of the time, the goal is to spend as...the difference. Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments...

... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...require a protein to be tagged, which limits these approaches to use with recombinant proteins. Purifying an...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

...interview or whether your receive the next grant you apply for. After all, the only value your work will have... slide, think about whether the same principle applies to other slides in your deck.  If someone asks ...stay tuned to our website and social channels to apply for our future events. In addition to this annual...

... exon skipping. Previous CRISPR exon-skipping approaches used two guide RNAs to remove a given exon from...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d...sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools available...

...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some... after less than a minute of exposure to the appropriate light. The photoactivatable counterpart to this...protocols to get your CRISPR experiment moving. Happy editing! References and Resources References Hendel...

... and remove a pathogen. Different isotypes also appear at different stages of an immune response. There... isotype of an antibody so you can select an appropriate isotype control. An isotype control has the same... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...teams around the world who combine engineering approaches and scientific know-how to design/build/test ...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...

...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...intermediate won’t just have nicks: it may have gaps or overlapping regions of single-stranded DNA. But as long as...of multicomponent assembly, as fragments with overlapping sequence homology can be assembled in a unique...

...infectivity. Next, the virus must assemble and have appropriate factors displayed on the outside of the particle... production, they can be helpful for certain applications and aid in ease of use. Our lentiviral guide...elements discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake...

...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this... this blog we will focus on those which can be applied in cell culture and animal models primarily. Fun...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

...guidance and structure. I quickly learned that my approach needed to adapt based on individual needs. For... and disengagement. By tailoring my leadership approach to each person, I was able to create a more productive...tension between two team members with differing approaches—one detail-focused and the other big-picture ...

...can firmly gain a handle on understanding and appreciating their biology. The importance of psychrophilic... the empty vector. And thus was born ELSA - our happy lil’ cold-tolerant E. coli! Additionally, we’re ...re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a...

...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system...multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate ...

...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation...but there are several hazards to navigate when mapping out a new project. We’ve shared some of the concerns...

... I will describe some of the many ways you can approach finding someone to give you advice and guidance...see someone with a great professional presence, approach them for advice on what makes them that way. Is...organization has a mentoring program, consider tapping into it and helping make it work by being an educated...

...work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but it’s toxic to tissues...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-...

...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective...that are not possible with traditional genetic approaches. In addition, the dTAG system is versatile and...update with vendor and catalog numbers. As we are happy to share aliquots of dTAG-13 in the meantime, please...

...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...rates for AAVs are generally high enough for this approach to work, although it’s less effective in vivo ... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of...method is free for you, and many labs are quite happy to share, labs that get high volumes of plasmid ...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced...times) into the genome. Make sure to choose an appropriate promoter to express your cDNA, depending on the...flavors of activators which can be used with this approach - read more about them on our CRISPRa blog! ...

...fluorescence that allow easy detection with the appropriate fluorescent illumination in in vivo, ex vivo,...of the most common tags currently used, are approximately 240 amino acids in size. The size of these proteins...Which tag should I use? When deciding on the appropriate tag, the experimental design should be the main...

... changes in neural activity when paired with appropriate fluorescent optics. Existing red fluorescent ...organisms such as E. coli and yeast. Because this approach uses standardized parts, it allows for greater...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...