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...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...  To start, the average health science startup requires a great deal more capital to get off the ground... your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking the necessary...

...Addgene Blog Mentoring for Scientists Series. The entire series and additional resources can be downloaded...responsibility of arranging meetings and setting the direction in a mentoring relationship falls on the shoulders...Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the "Forced Choice...

...communication. A great approach to fully understand and acquire competitive skill sets in a myriad of professions...job sites to find open positions and view their required qualifications and skill sets Taking the time...graduate students and postdocs are currently getting hired? Let us know what topic you'd like to learn more...

... Just enter the DOI of a paper and select your desired formatting style. This tool is great for generating...with others. The Web Importer plug-in lets you directly import papers from web pages and academic databases...the Zotero Connector, papers can be saved from Firefox or Chrome browsers with just one click. Papers ...

.... The tools are a lifesaver when your research requires cell culture and include a counter, lab timer,...Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a ...the app is quite helpful and provides a list of directions including which buffers to use and that indicates...

...BosLab and previous laboratory experience is not required. Our members are skilled with audiovisual media...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

...scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab mates or to... to discuss a potential new research direction you’d like to pursue? Undefined scope can lead to long ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

...truly believes that sharing speeds science, and our entire mission is to enable that process. Our repository... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, for example,...

...catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....

...label your favorite protein! While the target only requires a small epitope tag (FLAG is eight amino acids... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...when encoding multiple guides. While two guides require two arrays for Cas9, two guides can be streamlined...

...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...ability to focus, especially on days when I was tired, stressed, or rushing. I would spend the time quietly...

...it like winter tires in different conditions: the more flexible rubber of winter tires give a better grip...recombinant proteins for industrial or research purposes requires stable host cells (the simplest example would ...

...biological phenomena by generating an output which is directly proportionate to the amount of a given factor ...either factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena...type of cells the viral particles infect. This can direct your biosensor to specific tissues or cell (sub...

...transfect’, such as primary cells. They can also be directly injected into hard-to-reach organs, such as the...like replication incompetent viral particles, are required. We will be covering these safety measures in ...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...

...activity, scientists can use GECIs to watch neurons fire under the microscope. To add another tool to our...developed calcium indicators spanning nearly the entire visible spectrum (Figure 1). The GCaMP series (... yellow and red fluorophores, allowing it to be paired with green, yellow, and/or red sensors in multicolor...

...display-based directed evolution to create APEX2. They have now applied the same directed evolution technique... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...

...tropism. Briefly, different envelope plasmids can direct the production of virus with various tropisms.  ...therefore, reduces the number of plasmids that are required in the transfection step of the viral packaging...incompetent? Yes, as with lentiviruses, the genes required for γ-retroviral packaging and transduction are...

...to read a formulaic fiction story that doesn’t require any detailed retention of information or formulation...something verbally. Sometimes a group was just really tired and needed a slower, simpler presentation than I... I had planned; other times students were fired up and enthusiastic and needed a faster presentation with...

...RNA, a fast, strong protein denaturant would be required- something that broke down RNases before RNases... effective at isolating total RNA, this method requires a lot of time and depending on how many samples...without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

...because “it kinda sucked to work remotely. I was also tired of the contracting life where you do a little bit... was helping cover social media while the Hutch hired a full-time social media manager. “I usually start...magazine. Getting pulled in all of these different directions is sometimes at odds with the space needed to...

...Much of today's biological research requires a close examination of specific proteins within a system...tags, however, tended to be very large and often required the incorporation of a cleavage site and a post-purification...no cut or dried answer. Successful purification requires the tag to be accessible to the metal ions once...

...sufficient for certain patterning activities of entire tissue types or even organs. In frogs, ectopic ...electrical communication is briefly inhibited in the entire worm during regeneration, the worm creates two ... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...

...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on Women’s Health, emphasizes...gender in science and technology and currently directs the EU/US Gendered Innovations in Science, Health...

...with multiple inserts or internal mutations that require more than two sequencing reactions. In the more...to ensure uniformity of the culture. Sequencing directly from the glycerol stock saves us the step of purifying... Each sequencing service will have different requirements for volume and concentration, and not all providers...

...new job skill? Finish your thesis? Knowing your desired outcomes will help you assess if the coach you ...coach usually will be a clear communicator and make direct suggestions for improvement and change. Work ...relationship has been successful – what are the desired outcomes? Ask openly for clear information on...

...asymmetry in the core sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT...reversed.   Deletion: If the sites face in the same direction, the sequence between the loxP sites is excised...cerevisiae FLP-FRT recombination system is another site-directed recombination technology very conceptually similar...

..., comprised of PAS and GAF domains of iRFP713. Requires presence of heme oxygenase for chromophore formation...miRFP709 and miRFP670, pair with common PAS domain. Requires presence of heme oxygenase for chromophore formation...BiFC interaction is typically irreversible and requires some time for chromophore maturation, splitFAST...

...active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is... is expected to contribute to experiments that require high photostability, such as time-lapse imaging...Leopold Parts. This library delivers 2 randomly paired guides per construct and allows a reduced scale...

...photon 2. Energy transfer from the excited donor directly to the acceptor fluorophore--think of it as a ...fusions varies with transfection efficiency and any unpaired fluorescent proteins can contribute additional...Check Out Fluorescent Protein Biosensors Browse the Entire Fluorescent Protein Collection ...

...effectively utilize the host cell’s inherent homology directed repair (HDR) process following the double strand...break and introducing the tag through homology directed repair To introduce the fluorescent tag to the...on the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining...