We narrowed to 801 results for: MAK

1. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   ...just Tris buffer, or some other variations. Does it make a difference? Short answer: yes. This last step ...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   ...labeling of a subset of cells with the same genetic makeup, overcomes all of these labor-intensive and invasive...

3. Hot Plasmids and Viral Preps - July 2021

   Type

   Blog Post

   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   ...of course! You just need three plasmids to start making the AAVs you need for your experiment:  the packaging...

5. Sensing Neuronal Dopamine

   Type

   Blog Post

   ...also involved in reinforcement learning, decision making, and motor control.  What do we understand about...

6. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   ...is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By mining the treasure trove...

7. Addgene at the ASM Microbe 2016 Conference

   Type

   Blog Post

   ... technology. Addgene got several shout-outs for making popular screening libraries like Brie and Brunello...

8. Advice for Choosing a Research Project

   Type

   Blog Post

   ...After joining a lab, the next important decision to make is “What project will I work on?” In the short term...

9. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   ...influenced by signals it receives from the entire body, making it difficult to probe due to the lack of tools ...

10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    ...reproduce only in experimentally controlled conditions, making them far safer to work with than virions. The four...

11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type

    Blog Post

    ...helps to know the right people) -> How can you make a decision about something like this without sufficient...

12. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    ...template that contains your desired edit, and I want to make sure that I (and Addgene) don’t forget this in the...

13. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    ...enhance protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because ...

14. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    ...control and we are expanding our viral service to make these readily available to the community. We now...

15. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    ...gene expression of cells at a single time point, making it difficult to investigate how early expression...

16. New and Upcoming Viral Vectors - Spring 2019

    Type

    Blog Post

    ...example, overall brightness or on/off kinetics) that makes them suitable for different applications. We recently...

17. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    ...the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important...

18. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type

    Blog Post

    ... repository, each verified by sequencing, which makes the repository a convenient source of sequence data...

19. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

20. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    ... be able to expand upon the work and continue to make contributions towards this COVID-19 pandemic,” says...

21. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    ...edit, and access the affinity reagents they need to make their next big discoveries. We welcome you all to...

22. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

23. RUBY-Red Siliques

    Type

    Blog Post

    ...identification of these mutants will speed up my work and make it easier to study these mutants.   Fig. ...

24. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    ....” Fear not, there are many ways we’re trying to make MTA turnaround faster with technology transfer offices...

25. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    ...sentences - even if written in a clumsy language make it much easier for me to go back and finish the ...

26. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    ... small nuclear DNAs from binding NgAgo, it might make it difficult to use NgAgo to disrupt multiple targets...

27. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    ... curate, and distribute them all in the name of making it a little bit easier for scientists to conduct...

28. Another Pathway into Cells: iTOP

    Type

    Blog Post

    ...iTOP, especially in tandem with genome editing, may make primary cell work much more efficient and feasible...

29. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    ...much of the primary data one might require when making such a decision remains spread across literature...

30. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    ... like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped out, and comparing...

31. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type

    Blog Post

    ...interested in facilitating collaborative science, and making it accessible to all people, not just scientists...

32. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    ...early on introductions and literature reviews could make the months leading up to your defense less stressful...

33. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

34. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    ...interpreting human variation at large scales and making personalized medicine a reality,” Chiasson explains...

35. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    ...addition to thorough ethical considerations, in making sure that the research has been adequately proven...

36. Networking Follow-up: I Met a Person. Now What?

    Type

    Blog Post

    ...just one more meeting (in person or virtually) will make the relationship stronger.  If appropriate, invite...

37. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    ...transduction patterns of different AAV serotypes. To help make this easier for scientists, we now offer trial size...

38. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    ...tissue penetration than shorter wavelengths, which makes it possible to do things like in vivo imaging.   ...

39. Four Factors that Differentiate the Stem Cell Field

    Type

    Blog Post

    ...meeting, I've made some observations regarding what makes this group of researchers special: 1. Stem cell ...

40. 5 Great Apps for Lab Life

    Type

    Blog Post

    ...user searches, which is not very helpful, but does make it easy to skim the manual. Overall, it’s a great...