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  2. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ... which display sophisticated interactions with multiple other proteins. Again, little to no change in ... place to start. These tags are easy to use in multiple common applications. Due to their limited effects...

  3. Science Communication for Everyone

    Type
    Blog Post
    ... so on, differently than scientists would. For example, “manipulate” could mean “illicit tampering” to...founder of The Addictive Brain. Prior to this, he completed PhD from Philipps-University Marburg, Germany ...

  6. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ... to mind! Attendees consistently mentioned how pleased they were to learn about these exciting scientific...science, all you need is a great idea and a group of people willing to make that idea a reality! How do you...

  7. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their ...facilitating the exchange of not just the physical samples, but also the legal documentation that allows you...

  8. Let There Be LITE Plasmids

    Type
    Blog Post
    ...addition of histone modifiers. “We want to allow people to prove the causal role of specific epigenetic...combine with different effectors. I’d encourage people to get creative with them for their own uses.” ...

  9. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog...and Lukas Morgan There are many model organisms people are using for their research. In this blog post...

  10. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over...

  12. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...arms that flank the donor plasmid cargo are complementary to either side of the double-stranded break ...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

  14. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...are appying for diffrent types of positions (for example, bench research vs. project managment) your resume...a specific department and/or location. The more people you meet the better chance you have of finding ...

  15. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...microbiology, virology, and genetics - before going on to complete her Masters degree at Imperial College London ...ideas with others. This love of interacting with people and talking about science makes her the perfect...

  17. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments. Widening...successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular biology and metabolism...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag system, showing...Resources page. Here are some highlights from the past couple months: A new lentiviral CRISPR library from Alec...

  19. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein... However, this system is far from perfect. For example, If your fusion protein is expressed at low levels...

  20. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ... are leaders in the field of microbiology with multiple high-profile publications. My classmates consisted...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

  21. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...food pantry or community arts organization. Many people are surprised to learn that Addgene is officially...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...

  22. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in...

  24. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...Got You Here Won't Get You There: How Successful People Become Even More Successful, by Marshall Goldsmith...

  25. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...e.g. pLKO.1 - TRC cloning vector). Check out our complete guide to empty backbones for additional details...

  29. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...PCR product. If you need to decrease error rate, please use one of these methods to ensure your insert ...

  30. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...

  31. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...(on a schedule) I hope you all know by now that people get jobs at least 85% of the time because of relationships...pretty easy to make 30 minute appointments with people all over the world. Think about contacting alumni...

  32. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...

  33. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  34. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...and transgene expression (Zhou et al. 2017). For example, mutations in the RBE site reduce binding with ...AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different...

  35. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...representation is most easily understood visually. As an example, the curves from our recent amplification of the...

  36. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  39. Antibodies 101: Isotypes

    Type
    Blog Post
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

  40. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...inspires students and teachers to apply engineering principles to biological systems. Today, what started in... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub...

  41. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene...double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control...

  43. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...change to a slide, think about whether the same principle applies to other slides in your deck.  If someone...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

  45. Antibodies 101: Polyclonal Antibodies

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    Blog Post
    ...several different species are present. One common example is using pre-adsorbed secondary antibodies (which... other antibodies) in experiments that require multiple antibodies that each detect a different antigen...

  46. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

  48. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org...

  49. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... for photoswitchable kinases.  Below are a few examples demonstrated in Zhou et al. 1. Testing kinase ...Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells." Cell ...

  50. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...
Showing: 801 - 850 of 1011 results