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1. Hot Plasmids - February 2022

   Type

   Blog Post

   ...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a...

2. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

4. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   ... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...

5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...

6. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   ...input. Usually that function is in the shape of an S; there is a narrow threshold like a switch that turns...

7. Neurodegeneration Research Collection

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   Collection

   ...and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter...

8. CRISPR Plasmids - Tagging

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   Collection

   ...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...

9. Antibodies 101: Fab Fragments

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   Blog Post

   ....1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi ...

10. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ... guide AAV Viral Preps References  Chen, Y., Hu, S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston...

11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    ... to do once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide...

12. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...audience is expending to pay attention to the mode(s) of communication you choose.  Audience knowledge ...

13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure...

14. What's New in CRISPR - November 2021

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    Blog Post

    ...By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large...

15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...References Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability...

16. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used...

17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    ...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas...

18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    ...Together these two experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent...

19. The Future of Research Symposium Boston 2015

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    Blog Post

    ...Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective...

20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...