We narrowed to 339 results for: ADA

1. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   ...deaminase (evolved from a tRNA deaminase enzyme, TadA) to induce a A→G mutation at the target genomic ...

2. Plasmids 101: Using Transposons in the Lab

   Type

   Blog Post

   ...closer look at three different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac...

3. Important Considerations When Using AAVs

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   Blog Post

   ...Skeletal Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/...

4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

   Type

   Blog Post

   ...be at any one of many stages of synthesis and degradation. For this reason it is virtually impossible to...

5. Cancer and the Immune System: Deciphering the Relationship

   Type

   Blog Post

   ... radiation therapy (owing to contributions from Madame Curie), chemotherapy, and surgical removal of the...

6. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   ...fluorophores in relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-...

7. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   Blog Post

   ... a table listing some of critical differences (adapted from Zhou and Melton, 2008, [43]): Characteristic...

8. CRISPR 101: Multiplex Expression of gRNAs

   Type

   Blog Post

   ...with a uracil DNA glycosylase inhibitor with a degradation tag (LVA tag) to achieve specific point mutations...

9. CRISPR 101: Cytosine Transversion Editors

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   Blog Post

   ...J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-to-G•C ...

10. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    ... entered using our online form or by using a downloadable spreadsheet. Either choice can be used for deposits...

11. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    ...monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., 2024.   Once an antibody...

12. Delivery Methods for Generating iPSCs

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    Blog Post

    ...42. PubMed PMID: 21339765. Methods figures were adapted from this reference. 1. Malik, Nasir, and Mahendra...

13. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...CASANOVA system is versatile in that it can be adapted to regulate gene editing, turn on transcription...

14. Chemogenetics Guide

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    Guide

    ... 25587888 (Link opens in a new window) Coward P, Wada HG, Falk MS, Chan SD, Meng F, et al. (1998). Controlling...opens in a new window) Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ, Lee H-M, Sciaky N, Simmons...Shimojo M, Zhang M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020...

15. Adenovirus Guide

    Type

    Guide

    ...immunogenic because they activate both innate and adaptive immune responses in the host. More than 80% of...structural capsid proteins. Glossary Term Definition Adaptive immunity Specific and long-lasting immune response...

16. Science Guides

    Type

    Guide

    ...alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been modified ...

17. Promoters

    Type

    Guide

    ...transcribes genes encoding transfer RNAs (tRNA), the adaptor molecules that are responsible for bringing amino...

18. Cloning

    Type

    Guide

    ... website. Ligation Independent Cloning (Figure adapted from LIC protocol ) Back to Top Yeast-mediated ...

19. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...39322487 (Link opens in a new window) Matsushita, T., Okada, T., Inaba, T., Mizukami, H., Ozawa, K., & Colosi...