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We narrowed to 954 results for: APP

Showing: 321 - 340 of 954 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control  In...can also directly paste sequence data into the appropriate full and partial sequence cells. *Note: If you...descriptive plasmid name and match the name as it appears in its publication or as it is commonly known in...descriptive purpose focuses on the practical application of the plasmid and will help a scientist outside... account username. In order for the plasmids to appear in your Addgene account under My Plasmids, we need...

  2. Hot Plasmids February 2024

    Type
    Blog Post
    ..., its dimeric nature can interfere with some applications. Several labs have been working to produce monomeric... for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038...successful in primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants...proven itself a good option for essentially all applications. Addgene’s plasmid collection includes over ...clone (M2) has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here...

  3. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    .... We both had an applied science bent, so the engineering aspect of this job appealed to us. However, ...possibilities in life for employment?  It never happened – there was never a “moment”. But there were a...about continuing with the business. We ended up applying to a business accelerator freely stating that ...your thoughts with us in the comments below. We’re happy to answer questions about this transition (so far...

  4. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...contain rAAV. To minimize contamination, use appropriate decontamination procedures (Korte et al., 2021...surfaces that come into contact with the rAAV and apply common cell culture practices diligently throughout... toxic transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your...al., 2010). For certain in vitro and in vivo applications, you can consider using unpurified AAV from ..., 2017). You can learn more about common AAV applications by reading our Viral Vectors 101 series!  This...

  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...carefully) holding plates up to the light, where they appear as opaque dots (colonies should contain at least...only 1.5 ml of each are used). This solution is capped, swirled and tested for the correct temperature...and not spread out onto the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the...densities, then dispose of the plates that do not appear to be yielding well-spaced clones. For rapidly ...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

  6. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...uncontrolled cellular proliferation. We have appreciated for some time the causes of this uncontrolled...cellular machinery necessary for division. This approach also kills dividing non-cancerous cells, such ... University of California, San Francisco, is approaching the Ras problem by exploiting the different mutant...genes from the Ras signaling pathway. Its major appeal is that it enables the simple construction of vectors...any of these genes through several different applications such as fluorescent protein fusion studies and...

  7. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few...tips. Based on the discussions in the forums, it appeared that many researchers were having difficulty achieving... target site. Overall, 64% of researchers were unhappy with the new method and 65% wanted to wait until... the comments section below. Keep pipetting and happy genome editing! Check Out Other Argonaute Plasmids... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...lot of activities (try for a dozen!). The best approach may not be “let’s try to make this STEM activity...that would compliment the STEM curriculum. As it happened, I’d met some of the 8th grade science teachers...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....analogy that involves student participation, make it happen! How should we use CRISPR? How shouldn’t we use... I came out of the experience with a renewed appreciation of our science teachers’ difficult-but-rewarding...

  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first gene therapy for the treatment...AAV-based gene therapy treatment for a genetic disease approved by the FDA is for a rare eye disease and not another...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...medicine. References and further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The... after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period of 30 minutes...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...recap of the pros and cons of each antibiotic and happy selecting!   Antibiotic Resistance Stability...

  11. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...with an abundance of protocols and examples of applications from across disciplines now available. In this...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...and Stiles, 2022). Fourth, signal continuity can appear reduced in ExM, which could impact how you interpret...pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should...especially when paired with complementary experimental approaches. Since it arrived on the scene, ExM has helped...

  12. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...variations in gene expression, they found that for approximately 40% of the identified genes, the genes were ...lines from individuals of African descent have approximately 20% more single nucleotide polymorphisms (SNPs...ancestry-associated cancer dependencies are undercounted by approximately 20%. In some cases, this may lead researchers...map. Now, any data that came from CRISPR guides mapped to a SNP would be ignored. Working with Lia Petronio...8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, ...

  13. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...In this Plasmids 101 post, we’ll cover why this happens, how it might affect your work, and how understanding...rely on chance (or random diffusion) to make this happen.  There are several different versions of the plasmid...way to bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids...pestis using plasmid incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111...Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural Biology...

  14. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ... tangible and applicable to your situation.   Thinking Outside the Box: Team Approaches At some point ...required to develop new projects and test novel approaches, a necessary skill set for any scientist. In ...lab   Thinking Outside the Box: Individual Approaches Whether it’s finding the source of contamination...creative skills you have developed in the lab can be applied anywhere! References and Resources References ...

  15. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...to generate new pEcgRNA. pEcCas/pEcgRNA can be applied not only in coli K-12 strains, but also in E. coli...genome editing in microorganisms which could be applied to optimization of genome editing tools.   Jieze...engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of...research focuses mainly on the development and application of a multiplex genome editing system based on...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    ...move from lab to office will likely happen eventually. If you approach that transition dreading the change... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...thinking” in the position to which she was applying. I probably did not respond to her question as well then...adjustment period will be that much longer. If you approach the transition embracing the opportunity for growth...

  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop... tools we make available. This prioritization happens for good reasons. We began offering our viral vector...remains the more requested option, and jGCaMP8 appears to be growing in requests faster than jGCaMP7 (...viral vectors. This process builds on our former approach and involves reading primary literature, soliciting...redesigned Data Hub in 2022. It’s influenced the way we approach our viral vector educational resources, from eBooks...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...connection you made at the job fair when you formally apply for the position. This post on Business Insider ...re likely to find current job openings. A good approach is to do this first for companies at which you...connection. That way, once you’ve identified an appropriate job opening, you can send your contact an email...the assistance of recruiters for identifying appropriate job candidates. If you do receive such a call...those relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp...website invites you to input the target region (approximately 500bp is best) and select the organism. It will...different types of gRNA design software. When you are happy with your selection, ordering the sgRNA is simple...process, you should first develop primers to the approximately 500 base pair sequence surrounding your gRNA...

  20. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...exist in the real world and corrective approaches and appropriate controls are required to make this method... this technique has revolutionised the way we apprehend molecular complexes and is still a very useful...experiment. You can then choose the most efficient approach for your own system. The simplest and the most...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...
Showing: 321 - 340 of 954 results