We narrowed to 700 results for: BASE

...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...

...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...

... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...

..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...

...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

...their choice to share their fluorescent protein based research. This year, we put out another call for...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each...

...user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber...changes cytidines or guanines to the other three bases, which limits the level of variation that can be...

...and proud member of Crowdfight. He is currently based in Spain and works on climate change. You can follow...the coordinators at Crowdfight. She is currently based in Spain and works in the field of genetics and ...

...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...

...the PCR is complete, the product can be verified based on size by gel electrophoresis . Protocol Video ...hydrogen bonds are broken between the nucleotide base pairs. The initial denaturation step is commonly...temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending on whether you are using ...

... more than one person, we selected the finalist based on the first time of submission.)     We love...

...predicted deleteriousness of the amino acid change based on maps of protein structure. Sometimes this provides... years, we’ve hoped that large scale laboratory-based functional experiments that classify which variants...

...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

...Addgene depositor Alice Ting used yeast display-based directed evolution to create APEX2. They have now...without special equipment. What’s more, detection is based on color changes to the assay paper, so no special...

...modified) for optimal stability and efficacy.    Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

...critiques or endorsements. In contrast, journal-based peer review is usually a confidential process among...ensure a manuscript’s quality. But even journal-based peer review can be prone to biases or errors, with...

...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...