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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 712 results for: BASE

Showing: 321 - 340 of 712 results

  1. Antibody Production

    Type
    Collection
    ...determines ideal shipping conditions for each order based on the total items in the order, ensuring each item...

  2. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, ...powerful high-throughput screen to select variants based on performance metrics specifically for two-photon...phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... The HA frankenbody works just like an antibody-based probe, but is easier and cheaper to generate since...scFvs: Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic...blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about ...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...

  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

  11. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber...changes cytidines or guanines to the other three bases, which limits the level of variation that can be...

  14. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

  15. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...

  16. DNA Quantification

    Type
    Protocol
    ...accurately measure the concentration of a substance based on its absorbance, you need to know the wavelength...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ... LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET...Kit , ONE-GO Biosensors Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR :...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the...57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several advantages...delivery system for human patients. Multiple viral-based gene therapies have already been approved by the...
Showing: 321 - 340 of 712 results