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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 382 results for: Bre;

Showing: 321 - 340 of 382 results

  4. DNA Purification Without a Kit

    Type
    Blog Post
    ...method for you! And even if you’re not ready to break-up with your DNA purification kit, you can still...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR...

  11. CRISPR Guide

    Type
    Guide
    ...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...conversion in RNA or DNA without a DNA double-strand break Cas C RISPR As sociated Protein, includes nucleases...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    .... abscessus. A) Sth1Cas9 mediates double strand break at the target site, which results in either bacterial...
Showing: 321 - 340 of 382 results