We narrowed to 641 results for: CAR
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TypeBlog Post... that your request is ready to order. An “Add to Cart” button will appear with the request in your Addgene... be charged once you have added the prep to your cart and checked out. Prices for Packaged on Request...
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What's New in CRISPR - September 2019
TypeBlog Post...genomic loci of interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector...sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...Essen University of North Carolina at Chapel Hill University of Illinois Chicago ...Consortium collection enables research on solute carriers (SLCs) for medical research Recently, the Michael... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post..., R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating that ...and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...dendritic cells. These genetic tools were pivotal in carrying out my studies, and getting them from collaborators...has since studied and worked around the world, carrying out her undergraduate work at the University of... -
Tips for Improving Your Next Manuscript
TypeBlog Post.... Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and...consisted of graduate students, post-grads, and early career scientists. Over the course of the class, the moderator... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...data found in full-length publications. As early career researchers, we wondered how we could make the ... blogger Girija Goyal! Girija Goyal is an early career researcher and cofounded ReFigure with Joe Akin... -
Advice for Choosing a Research Project
TypeBlog Post... stone into the next professional stage of your career by connecting you with people of similar interests... research question that people (especially you!) care about. Have you critically evaluated your plan... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...wall synthesis Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal...optimal efficiency. Beware of satellite colonies! Carbenicillin is more stable than Ampicillin and can be used... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions using slightly different Cas... Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Jan; ... -
New Viral Vectors - Winter 2025
TypeBlog Post...Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype ... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post... new particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both ... possible, just to increase the viral vector’s carrying capacity. In order to allow the viral vectors ... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...Bachle Listen to the Caspex podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics... to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group from the University... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...LTRs). After recombination, one resulting plasmid carries the transgene of interest (aka the viral genome...picking the smaller colonies which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed... -
RUBY-Red Siliques
TypeBlog Post...pigments primarily found in plants in the order Caryophyllales, such as cacti and beets. Betanin belongs to... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post... MBP tag can be removed using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag...for affinity purification (see plasmid map and cartoon below). Including biotin in your culture and inducing... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now... below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only the... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...institute where their typical workdays will be carried out until the end of the appointment. The visiting...don’t forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it... -
K. phaffii: Rising to the Occasion
TypeBlog Post...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K.... -
Celebrate National Book Month with Addgene
TypeBlog Post...compromising on the relevant science." - Randall Carter, Contracts Administrator Inferior: How Science ...Story by Angela Saini "This book is a smart and carefully presented case for better more diverse science...