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We narrowed to 644 results for: CAR

Showing: 321 - 340 of 644 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ... to try this at home in their own lab: be very careful with the type IIS restriction endonuclease of choice... PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision Cloning...

  2. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...covered in a future post in this series). Members carry on back-channel or side conversations within the...and suggestions. Consider offering rewards (gift card, afternoon off, etc.) Act on information to make...

  5. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...genomic loci of interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector...sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...

  6. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... that your request is ready to order. An “Add to Cart” button will appear with the request in your Addgene... be charged once you have added the prep to your cart and checked out.  Prices for Packaged on Request...

  8. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...dendritic cells. These genetic tools were pivotal in carrying out my studies, and getting them from collaborators...has since studied and worked around the world, carrying out her undergraduate work at the University of...

  9. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    .... Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and...consisted of graduate students, post-grads, and early career scientists. Over the course of the class, the moderator...

  11. Advice for Choosing a Research Project

    Type
    Blog Post
    ... stone into the next professional stage of your career by connecting you with people of similar interests... research question that people (especially you!) care about. Have you critically evaluated your plan...

  12. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...wall synthesis Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal...optimal efficiency. Beware of satellite colonies! Carbenicillin is more stable than Ampicillin and can be used...

  13. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions using slightly different Cas... Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Jan;  ...

  15. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype ...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...pigments primarily found in plants in the order Caryophyllales, such as cacti and beets. Betanin belongs to... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ... new particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both ... possible, just to increase the viral vector’s carrying capacity. In order to allow the viral vectors ...

  19. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ... MBP tag can be removed using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag...for affinity purification (see plasmid map and cartoon below). Including biotin in your culture and inducing...
Showing: 321 - 340 of 644 results