We narrowed to 649 results for: CHI
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TypeBlog Post...119741 AAV5 AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742 AAV5... AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...
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Hot Plasmids and Viral Preps - September 2021
TypeBlog Post... examine biosynthetic pathways A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use...-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell ... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...zebrafish embryo over an 80 minute time course. By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...accompany him to YNP the following year. Geoff Schiebinger, another Quake Lab member joined for half of ...in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due to the 3,471 square... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing ...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...Glick from SnapGene! Ben Glick is President and Chief Scientist at SnapGene, and also a Professor of Molecular...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post... research, we hope we can help all researchers achieve happiness in their careers regardless of their ...by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find One? ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at...the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent... -
K. phaffii: Rising to the Occasion
TypeBlog Post...pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to create gene-edited...claim has yet to be independently verified, the watching world erupted in controversy. Briefly, there are... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...Alzhiemer’s disease (AD). Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...resting state and its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor...inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...free interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific...Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...complicated (and again, expensive) the different machines are, and how many regulations are in place to ... on the Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can... -
Cultivating Community Science at BosLab
TypeBlog Post...was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...you volunteer to do science outreach and teach children about science? Or perhaps you have presented in...specifically to assist graduate students in job searching. Start your career planning early, start now Don't... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post... 3) produces bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results...however, you may need to run the gel for longer to achieve good separation of larger DNA fragments. I hope...