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We narrowed to 570 results for: Cit

Showing: 321 - 340 of 570 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ... occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas and Cas-like...Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol...

  3. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9... in order to call that gene a hit.  This is an exciting time for functional genomics, with an ever-expanding...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...shown to offer high activity and unparalleled specificity in genomic targeting design flexibility. The ...cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently ...

  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...original enzyme, SNAP-tag and CLIP-tag show good specificity for their own ligands with minimal cross-reactivity...Protein-based tags typically provide better labeling specificity than short peptide tags like tetracysteine (Jing...

  9. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...final plasmid pages and scientists will be asked to cite this article when using your plasmids. You can submit...Deposit Spreadsheet More Data for You: Find Articles Citing Addgene Plasmids Resources on the Addgene Website...

  10. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...pattern of labeling, this is supportive of their specificity for the protein. A challenge to this approach...target with very low expression, due to induced toxicity caused by the protein of interest. HLDA workshop...

  12. Deep Dive: qPCR

    Type
    Blog Post
    ...of probes/primers often offer higher allelic specificity, due to the need for the target:probe/primer ...Chemistry Background noise Probe Allelic specificity Can be multiplexed Single amplicon validation...

  14. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...cleavage site, but this system probably have better specificity. A good reference is Ran et al. (2013). As ...RNA-Guided CRISPR CAS9 for enhanced genome editing specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016...

  15. Lentiviral Vector Guide

    Type
    Guide
    ...-term expression in a host, and exhibit low immunogenicity. This guide contains many viral vector-specific...of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid — contains packaging genes gag...popularity to further enhance both infectivity and specificity. Pseudotyped lentiviral vectors are common in...the target cell population and reduce cellular toxicity. Common Uses of Lentiviral Vectors Due to their...lentiviral vectors. Glossary Term Definition Immunogenicity The ability of a molecule or substance to induce...

  16. Plan Your Experiment

    Type
    Guide
    ...High-fidelity Cas enzymes increase specificity. Dual-nickase approach increases specificity but is less efficient....PAM sequence will have the highest specificity. To increase specificity, you can also consider using a high-fidelity...

  17. Chemogenetics Guide

    Type
    Guide
    ...ligand-gated ion channels (LGICs) that can either excite or inhibit neuronal signaling. PSAM-based LGICs...generates light that activates the opsin. The opsin may excite or inhibit the neurons expressing the LMO, depending...promoters in chemogenetics plasmids Promoter Cell Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68 Microglia...tissue, and this must be considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors...

  18. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...stable long-term expression in a host and low immunogenicity. This guide contains many viral vector-specific...shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid — contains packaging genes gag...glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells. Proceedings...and the eventual cause of serious diseases. Immunogenicity The ability of a molecule or substance to induce...

  19. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...transfer time. Be aware that the transfer works by electricity, so long transfer times increase the heat of ...from 1:100–1:1,000. If your antibodies come from ascites fluid, try a dilution range from 1:1,000–1:100,...

  20. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...We are excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   ...
Showing: 321 - 340 of 570 results