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We narrowed to 573 results for: Cit

Showing: 321 - 340 of 573 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...shown to offer high activity and unparalleled specificity in genomic targeting design flexibility. The ...cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently ...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...original enzyme, SNAP-tag and CLIP-tag show good specificity for their own ligands with minimal cross-reactivity...Protein-based tags typically provide better labeling specificity than short peptide tags like tetracysteine (Jing...

  6. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...final plasmid pages and scientists will be asked to cite this article when using your plasmids. You can submit...Deposit Spreadsheet More Data for You: Find Articles Citing Addgene Plasmids Resources on the Addgene Website...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...pattern of labeling, this is supportive of their specificity for the protein. A challenge to this approach...target with very low expression, due to induced toxicity caused by the protein of interest. HLDA workshop...

  10. Deep Dive: qPCR

    Type
    Blog Post
    ...of probes/primers often offer higher allelic specificity, due to the need for the target:probe/primer ...Chemistry Background noise Probe Allelic specificity Can be multiplexed Single amplicon validation...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...cleavage site, but this system probably have better specificity. A good reference is Ran et al. (2013). As ...RNA-Guided CRISPR CAS9 for enhanced genome editing specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...transfer time. Be aware that the transfer works by electricity, so long transfer times increase the heat of ...from 1:100–1:1,000. If your antibodies come from ascites fluid, try a dilution range from 1:1,000–1:100,...

  13. CRISPR Guide

    Type
    Guide
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...targeting multiple genomic locations or enhancing specificity, efficiency, or flexibility of the Cas enzyme...Vectors Engineering the Cas9 Endonuclease CRISPR specificity is determined by both the gRNA sequence and the... design, engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the...many modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s...cleavage of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands...fidelity of Cas9 Researchers have also enhanced specificity by engineering Cas9 into high fidelity Cas9s ...

  14. Antibody Guide

    Type
    Guide
    ... of 10 Antigen Specificity Antibodies are available in two types of antigen specificity: monoclonal, meaning...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a specific... Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization...variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to antibody..., and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...isotype-specific or even isotype subclass-specific. This specificity can be used to ensure secondary antibodies bind...cost-effective. They are useful when epitope specificity is not a concern or in cases where you may want...

  15. Adenovirus Guide

    Type
    Guide
    ...due to their well-established methods, relative simplicity of production, and higher titers. However, the... and E4. These modifications aimed to lower immunogenicity issues observed with first-generation rAdV ...vectors and further increase the transgene packaging capacity up to ~10.5 kb. The deleted E2 and E4 regions ...lines. Second-generation rAdV vectors were shown to elicit less immune response when administered in vivo,...vectors are also referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences...of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector. pAdEasy-2 does not contain...such as transduction efficiency, tropism and immunogenicity. For example, Ad5/35 and Ad5/26 combine the...

  16. Optogenetics Guide

    Type
    Guide
    ...Chlamydomonas reinhardtii . Channelrhodopsin-1 (ChR1) is excited by blue light and permits nonspecific cation influx...physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic ... as light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements...are some key factors to consider: Optogenetic excitation or optogenetic inhibition. First things first...experiment? Depending on your answer, you’d pick an excitatory or inhibitory opsin, respectively. Color of activation... EJ, Tian Z, et al. 2014. Independent optical excitation of distinct neural populations. Nat Methods. ...Finkelstein J, Paz JT, et al. 2011. Neocortical excitation/inhibition balance in information processing ...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...DNA synthesis but further limits the packaging capacity of rAAV vectors to ~2.4 kb. Figure 4: Examples...ability to target specific tissues and cells, and capacity for long-term gene expression. These features ...other viral delivery systems due to their non-pathogenicity, ability to efficiently deliver genes to a wide...brain structure due to their small size, low immunogenicity, and limited ability to spread after injection...capsid is challenging due to its limited packaging capacity, especially if any additional regulatory elements... information. Split AAVs to increase packaging capacity Another strategy to deliver cargo too large to...Engelhardt, J. F. (2001). Expanding AAV packaging capacity with trans-splicing or overlapping vectors: a ...

  18. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...We are excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   ...
Showing: 321 - 340 of 573 results