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We narrowed to 824 results for: EED

Showing: 321 - 340 of 824 results

  1. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...industry research, and to get advice on how to succeed in your own career. One pro tip from Crystal - ...that different people have different management needs. How Crystal developed her management skills - training...leveraging your unique skills set while also adapting to needs as they arise. Tips on applying for industry jobs...

  2. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... lattes and espressos. The energy boost is well needed to keep up with all the awesome talks, symposiums... order to help scientists find the plasmids they need for their research, show labs how they can benefit...their constructs to the repository, and getting feedback from our community. It has been great to hear ...

  3. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...experiment.  It will dictate the amount of DNA you need, and at which level of purity. Based on these premises...available kits and DNA for transformation doesn’t need to be as pure as for other uses.   Cloning grade...backbone. Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian cells? Use ...

  4. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...it, or determine its expression level without the need for a custom antibody. There is one major caveat...likely to alter a protein’s properties. Why do we need new epitope tags? Short epitope tags like FLAG, ...for both applications. To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions...

  5. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...controlled by a neuron specific promoter or you could breed your Cre-dependent mCherry line with a separate ...consuming. Instead, a quick way to get the results you need would be to inject your Cre-dependent mCherry mouse...therefore mCherry will only be expressed in neurons. Indeed, this AAV provides you with a means to specifically...

  6. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...are obtained by independent audit, and companies need to pay to obtain them. There’s a database of labels...a cultural change in how we approach waste that needs to be supported at all levels. Disposables are an...Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years ago that we...

  7. Editor's Choice, August 2016

    Type
    Blog Post
    ...Up: First Impressions of NgAgo In particular, we need to thank Pooran Dewari who wrote this month’s most...news article in Nature. Our take home: users likely need more time to optimize and troubleshoot the system...as each one has something great to offer, I just need to get back to emailing (hopefully non-vacationing...

  8. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...important foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and...cloned and prepped in E. coli. However, they also need to be able to replicate in Agrobacterium to ensure...promoter, allowing it to be removed and replaced if needed. In total, three MCS regions permit multiple regulatory...

  9. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences in March,...understanding and treatment of cancer. It is clear that one needs to capture a diverse range of CTCs to cope with ...SpyLigase system for the first time? Howarth: The Proceedings of the National Academy of Sciences paper [published...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold change in affinity ... were then further mutagenized and screened for speed and voltage sensitivity. This hierarchical screen... mutations for improved voltage sensitivity and speed, and an endoplasmic reticulum export motif and trafficking...like co-IPs and ChIP, and more - all without the need to develop custom antibodies. Unfortunately, while...autofluoresence and photobleaching, as well as the need for external light activation. In order to overcome...excite a fused fluorescent protein, eliminating the need for an excitation light source. Instead, coelenterazine...

  12. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... allow ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation...excel (and thus feel grateful) and identify areas in need of improvement. These unrelated ideas might hold...discoveries often emerge when we allow ourselves the freedom to explore beyond the expected. By widening your...

  13. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...conditions, and everything in between – now all you need are some of your favorite proteins purified to carry... bound to the glutathione resin, eliminating the need for an extra clean up step of the tag – a handy ...maybe try MBP if you are having solubility issues!). Need a GST antibody? Addgene has you covered! His His...

  14. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed in some way before...help you calculate how much insert and backbone you need for different types of cloning methods, such as ...help you choose the best CRISPR software for your needs 5 great apps for lab life  Sharing your lab protocols...

  15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...system is composed of a promoter-less reporter which needs to integrate within an exon of a gene to generate...gene to turn on expression. Enhancer traps do not need to be integrated within an intron or exon of a gene...directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame, in ...

  16. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ... through the NIH Common Fund, emphasizes that we need more standardized methods for evaluating CRISPR ...to move the CRISPR field forward. To do that, we need to learn much more about cellular DNA repair pathways...base editors, which alter genomic DNA without the need for cellular HDR.   Having had time to digest my...

  17. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...Scientific publications are a nice to have, not a need to have Must be ready to change projects frequently...haul Limited movement in one company, likely will need to move around every 3-6 years—a short and exciting...you focus on the transferable skills that you will need to get there. Additional Resources on the Addgene...

  18. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... hosts you intend to use, and whether or not you need to consider your plasmid's compatibility with one...same group should not be co-transformed, so if you need two plasmids for an experiment, make sure they have...have not started to die off. Have any questions? Need something clarified? If so please, post in the comments...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...intrepid researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor...giving an idea of the stiffness the fibers would need to be, as well as other features necessary for implantation...implantation of fibers, indicating the system was indeed stable for continuous monitoring. On the gut side...
Showing: 321 - 340 of 824 results