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We narrowed to 520 results for: HA tag

Showing: 321 - 340 of 520 results

  1. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...can also be packaged into AAV. NmeCas9 has the added advantage that it can be turned off by anti-CRISPR... 16, 2020 by Beth Kenkel.  CRISPR genome editing has quickly become a popular system for in vitro and ...packaging capacity of AAV (~4.7 kb). While this approach has been proven feasible, it leaves little room for additional...the number of targetable loci, and truncated Cas9 has much lower efficiency than its wild-type counterpart...daunting, it’s actually a challenge that the field has overcome with split AAVs. In general split AAVs break...

  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...quantified by cell transduction assays. Wild-type AAV2 has been reported to have a near-perfect physical-to-...performed on all new viral vector preparations. Addgene has used this method to generally validate our viral ... Material (AAV SFM), which is an AAV sample that has been quantified by 16 labs around the world and can...Hindson et al., 2011, Hindson et al., 2013), which has been shown to be more precise and more repeatable...2011): 8604-8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL,...wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing viral capsids is typically...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...measuring DA and other neurotransmitters in the brain has been in vivo microdialysis of extracellular fluid... dLight1 dopamine sensor In 2018, Lin Tian’s lab has developed dLight1, a molecular tool to detect and...here! The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy...collects the perfusion liquid for quantitation. The advantage of this method is that it can be performed in ...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...version of PubMed.  Key features This new version has an improved search algorithm which was trained on...your search terms. Key features Semantic Scholar has several ways to focus your search results. A bar ...paper’s entry, the number of citations that paper has is displayed at the top of the page, as well as the...saved to a Semantic Scholar library. You can add hashtags to library entries, which can help you organize... papers are published, similar to Facebook or Instagram. Key features Setting up a personalized feed is...

  5. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...power of science to improve the world around us has long been known; ensuring all have access to this power...information becomes easier to access by the day, it has never been more crucial to understand just how we...into a summer school course at the University of Otago’s Department of Science Communication the following... The Owheo building, home of the University of Otago's Science Communication Department The power ...Alex Gilbert is a PhD student at the University of Otago. Studying in the same Department of Science Communication...

  6. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...relationship, but if you don’t take this past the chatting stage you will rarely make any change in your skill sets... the helpful accountability that is one major advantage of working with a mentor.The responsibility of...graduate student – you get the idea. There are many advantages to being a Mentor: Learn other perspectives ...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ..., that informs us that this antibody has specific binding and has a strong signal. We call the unique ...strong signal has a shorter exposure time than the negative control, and a weak signal has a longer exposure...rAbs are a relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression...

  8. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...   Fig. 1: SpCas9 has sparse PAM coverage in the human genome. SpRY Cas9 has PAM sites covering the... a PAM with no sequence constraints, or at least has looser or different requirements. Luckily, there ...alternative PAM requirements (Zhao et. al, 2023). xCas9 has been shown to be less effective at NGH PAMs, though... PAM requirement, and how these proteins are advantageous in today’s genome editing landscape. The need...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...easily assemble multiple DNA fragments. This system has been used to construct plasmids for use in model ...Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized for its biotechnological... encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators...GEVIs) are proteins that detect and output the voltage changes in neural activity when paired with appropriate...appropriate fluorescent optics. Existing red fluorescent voltage indicators, however, fall short compared to GFP-based...containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the...amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin GEVIs. With...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...industry just wasn't for me, and as has been highlighted recently it has become more difficult to attain ...interesting and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic...because it is a small and dynamic company and so it has the flexibility of a ‘start-up’. I was surprised ...schedule.  I really love my highly varied role, it has allowed me to travel and to share my passion for ...everyone. My career path may seem quite unusual but it has allowed me to try out working in many different sectors... still feel now, that it is important to take advantage of the fact that science is a global language ...

  11. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...host cells immediately prior to their transduction has been shown to increase AAV transduction efficiency... such cationic reagent that is commonly used and has been shown to increase transduction efficiency by...single-use aliquots. Never use a polybrene solution that has been thawed more than once. Fibronectin is another...polybrene (e.g., hematopoietic or primary cells), and has been shown to increase transduction efficiency by...packaging cells after 1–3 days of selection. A lower percentage of surviving cells may be the result of plasmid...

  12. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Griesbeck adds that this color change has a particular advantage for observing events taking place inside...Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators of...Maarten Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...

  13. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... techniques is a speculation of yesteryear which has been realized recently. Science Fiction (SciFi) writers...even before the discovery of genes themselves.  How has scientific progress in genetic editing impacted the...technology on various non-scientific platforms, CRISPR has also transcended into the world of SciFi. This is...and most important genres of literary fiction. It has always served as a vision of the future and to make...and agriculture, its portrayal in popular fiction has been largely negative. But these negative depictions....1126/science.1258096 Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher... movie version (2016) stars Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing ...

  14. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... ddPCR.) qPCR Quantitative/real-time PCR (qPCR) has been used for copy number assays for years. qPCR ...and quencher to quantify specific amplicons.  Sybr has high background noise, due to the large amount of...AT-rich DNA and the length of the amplicon and it has a higher rate of false positives (Colborn, 2008; ... used to accurately call copy numbers, though it has been shown to identify trends correctly (Muhamad,...Muhamad, 2011;  Providenti, 2006). Its biggest advantage over Taqman is price point, which should be carefully...

  15. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...Not the case, as I’ve discovered.) As someone who has now seen both sides of the postdoc experience, I’... lab is closely involved with clinical trials or has ties to industry, the drug discovery process can ...Genentech, will not. The point is that each company has a unique approach, and some might suit your needs...currently a Postdoctoral Scientist at AstraZeneca. She has a background in cell and developmental biology and...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested...

  16. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...community has access to all of the latest resources? In response to this challenge, Addgene has launched... why recent work in the field of cancer research has focused on picking apart the complex cellular pathways...at Vanderbilt University in Nashville, Tennessee has already demonstrated that a number of novel small...ensues, traditional chemotherapies have taken advantage of the universally-dividing behavior of cancer...

  17. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ... of the gel. For small proteins, use a higher percentage of acrylamide to increase migration resistance...improve separation. For large proteins, reduce the percentage of acrylamide. To further improve resolution,...

  18. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...attributes (which turned into www.fpvis.org).  That page has been very useful over the years, and when researchers...system that allows users to see when a given page has unmoderated changes and to view previous versions... online collections of FP spectra. Every protein has a page with all the information in the database relating...of FPbase FPbase is still very much in the early stages of development, and there is plenty of room for...

  19. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...birth several weeks previously. While this claim has yet to be independently verified, the watching world...Kingdom’s Nuffield Council. While the Nuffield Council has recently published a report on the social and ethical...considerations, in making sure that the research has been adequately proven to be reasonably safe and ..., in vitro research, animal models, and several stages of human clinical trials. This model also demonstrates...

  20. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...easy. Most of our equipment has been donated by local companies and BosLab has grown into an organization... biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules... entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig is using ...
Showing: 321 - 340 of 520 results