...the Vancouver SciCATs. Follow her on twitter @Point_Mutation, and @SciCATSyvr. Additional Resources on ...

...of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1, Wiley, 25...

...epitope of the target protein. However, small mutations in the DNA of the hybridoma can occur, known as...

...are two main experimental avenues (that are not mutually exclusive) commonly used in optogenetics: 1) electrophysiology...

...knockout )? Generate a specific mutant allele of a gene ( point mutant )? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the... type or organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual) gRNA targeting... a particular gene, such as generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base...knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce expression... the N-terminus are targeted since frameshift mutations here increase the likelihood that a nonfunctional...of NHEJ. In HDR gene editing experiments, most mutated alleles will not contain the desired edit, as a...

...Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre None ...-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO EYFP...Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55650 pAAV-hSyn Con/Fon EYFP...recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137119 pAAV-EF1a-Con/Fon-...Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55645 pAAV-hSyn Con/Fon hChR2...Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137148 pAAV-nEF-Con/Fon-Arch3.3...recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 177669 pAAV-nEF-Coff/Fon ...

...repair (BER), the cellular process that catches mutated U’s in DNA and ideally converts them back to Cs...

...base pairs 18 and 20.   Pro tip! The Taylor lab mutated the residues in this loop to produce SuperFi-Cas9...

...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive... and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning ...Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein RBD mutations for comprehensive...Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of free access articles...Scanning Library - Library of Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2...

...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

...infectivity across a broad range of cell types. The mutant AAV-DJ8 displays the properties of AAV-DJ, but ...

...somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed... create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

... action and application of Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, ...

...levels mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1 Cre expressed at low levels mutant TEF1 Yeast...levels mutant TEF1 Yeast Piper 60933 pPL5628_TEF1*-Cre_MET15 Cre expressed at low levels mutant TEF3 Yeast...alternative Cre recombinases that recognize specific mutant loxP sites, VloxP and SloxP sites respectively....Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding domain CMV Mammalian Green 12497...12497 p274 pCMV-CRE-M-AR(T) CREM fused to mutant AR ligand binding domain CMV Mammalian Green 12529 pBS...different types of target sites available, both mutant and wild type. The ability to manipulate the number...pAAV-CA-FLEX Cre activates gene of interest, using 4 mutated lox sites AAV Uchida Cre Reporters and Tools In...

... for use in cancer signaling studies, including mutant, knockdown, and overexpression constructs. Each...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...Meyerson , and Root Labs containing wild-type and mutant alleles described in the initial Target Accelerator...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2...the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect ... To enable monosynaptic spread of this deletion-mutant rabies, the essential G protein is provided to ...2007a. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932...

... protein component of MMR) mutant to inhibit MMR PE5 - PE3 plus MLH1 mutant to inhibit MMR PEmax - optimized...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 - introduction of five mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

...bright mutant of Renilla luciferase (Rluc8) through an Ala-Glu-Leu linker to the circularly permuted Venus...enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly ...Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow for increased...Renilla CMV Mammalian expression renilla luciferase mutant Rluc8, which has increased stability and light ...

...truncated at both the N and C terminus and induces mutation at rates much higher than the parental vectors...generation architecture has been shown to increase mutation induction when using TALENs. The FokI domains ...homodimeric or enhanced heterodimeric (ELD, KKR mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster . Zhang...

...fluorescent marker. Injection into a conditional mutant mouse brain that expressed Cre recombinase only...