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We narrowed to 765 results for: Mos

Showing: 321 - 340 of 765 results

  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is...resistant to CcdB, as the native ccdA will be present. Most common cloning strains of E. coli do not contain...ever more important to ensure that you choose the most appropriate method for your own experiments. Please...

  2. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...gives you access to hard data about what steps are most likely to fail. Our laboratory manager would definitely...interested to see which part of her procedures are the most failure prone. In an academic lab, a similar situation...capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied to ...

  3. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...requested in the call. Step 2: Develop a research plan Most grant applications restrict the space available ...more advice on writing a grant proposal. "Murder Most Foul: How Not to Kill a Grant Application" by Vid...process? What types of writing feedback do you find most helpful? Have a great grant writing tip? Share it...

  4. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...allowing everything to stay in the correct order. Most Fragmid components are available for request on ...plasmid a name and generate the vector file. Ta-da! Most (180/181) Fragmid components are available in the... be using more than six or seven components — in most cases, that's making two or more plasmids — it may...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample and...the least-abundant protein and working toward the most abundant. If the abundance of your targets isn’t...maximize protein retention. Following this step, most PVDF membranes recommend re-wetting with methanol...

  6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent... is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working to develop CRISPR-based...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...“goldilocks antibody.” Antibodies are one of the most commonly used research tools in scientists’ pockets...kept in mind our own lab’s purposes. The Wu lab mostly uses whole-mount IHC techniques on rodent brains...

  8. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...T-cells were transferred back into the infants. Almost all patients have achieved persistent immunological...distinguish AAVs as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...

  9. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...how molecular biology impacts worm locomotion. Our most recent models of C. elegans molecular biology are...been incorporated as a non-profit entity and so far most of their funding has come from kickstarter and fundrazr...everyone knows the internet loves cats! This is mostly a project to get our computer folks at the makerspace...

  10. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... be difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab...though expressing the chromoproteins from the chromosome could get around this problem. There is no single... “ideal” chromoprotein plasmid that combines the most desirable features: intense color, fast maturation...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...because you will be part of a team in industry with most team members giving oral presentations, you will...to work on later. For instance, AstraZeneca posts most of its positions all at once at the end of February...method of posting varies between companies. Like most jobs, it does help if you know someone who’s already...

  12. Of Myc and Men

    Type
    Blog Post
    ...the Myc tag to the world, it has become one of the most commonly used tags in molecular biology. And the...we all really using the same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody...design experiments. It turns out that even with the most popular antibodies, context is key.    References...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...point during this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite...protein function in the human body?” … and probably most importantly… "how do we know this?” Over the past...represent one aspect of the protein very well, it will almost certainly fail to convey other aspects of the structure...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...reference sequence. The five contigs with the most total reads are most likely to correspond to the contaminant...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...production site to its final destination. As an example, most secreted proteins are produced in the endoplasmid... selected FPs need to be sufficiently separated, most commonly FPs with red and green wavelengths, respectively...microscopes the detection limit is 200 nm. The size of most proteins is below 10 nm and therefore colocalization...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...an extra wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰!...recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes ...

  17. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...genetic screening experiments”, it is certainly the most robust. In this blog post, we will discuss how CRISPR...libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are provided at a concentration... of next-generation sequencing. Representation - Most libraries contain 3-6 gRNAs per target gene, and...

  18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... we’ll break down the mechanism of action of the most common antibiotic resistance genes and which applications...bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin...?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies? Ampicillin Fair...

  19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...the world encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier ...placing orders per day across regions, with Europe most impacted and China least impacted. The inferred ... China COVID-19 date range is, they could be the most impacted or least impacted. These results highlight...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...response. At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and...limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot... is back, better and safer. It is now one of the most active and exciting areas of science. This is hopefully...
Showing: 321 - 340 of 765 results