We narrowed to 403 results for: NAM

1. Magnetic Control of Proteins: More than a Dream

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   ...response to the magnetic field (Figure 2); they named this variant MagLOV. Andrew was thrilled to see ...

2. 3D Printing Meets CRISPR Cas9

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   ...their flexible and jiggly nature suggested the dynamic nature of a protein. The Bender’s popularity was...

3. Plasmids 101: Degron Tags

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   ...for degradation. In addition to having the coolest name of all the tags reviewed here, the SMASh tag does...

4. Pooled Library Amplifications

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   ...Pooled Libraries at Addgene.  Library Type Name Number of Plasmids Variable Region Size DNA...

5. Hot Plasmids and Viral Preps - May 2021

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   ...providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our recombinase-dependent...

6. Light Sheet Fluorescence Microscopy

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   ...pioneered by Voie and colleagues and originally named orthogonal-plane fluorescence optical sectioning...

7. Plasmids 101: Multicistronic Vectors

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   ...manipulated to express more than two genes. Plasmid Name Multicistronic Element Expression Type MSCV-IRES-EGFP...

8. Tips for Using BLAST to Verify Plasmids

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   ...result should match, enter the common or scientific name into the Organism box. This small piece of information...

9. Simplify Cloning with in vivo Assembly

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   ... your primers and template. Amplifying the ‘unamplifiable’ Check your amplification by gel electrophoresis...

10. Plasmids 101: Secondary Nanobody Toolbox

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    ...Nanobody Plasmids Structural comparison As the name suggests, nanobodies are smaller antibodies.They...

11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...? The available filtering options now change dynamically based on the type of material and specific search...

12. SciComm: Taking Science to Elementary and Middle Schools

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    ...teachers, who work hard maintaining a current, dynamic curriculum, would be hard-pressed to maintain the...

13. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...Cross-Linkers Chemical cross-linkers work by (as the name suggests) cross-linking cellular proteins. Cross-linking...

14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...into bacterial genomes. This new capability was named INTEGRATE (Insertion of Transposable Elements by...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many thanks ...

16. CRISPR 101: RNA Editing with Cas13

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    ...and identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing ...

17. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...repository has played in these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth...

18. Viral Vectors 101: The Retroviral Lifecycle

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    ...have their own unusual sort of virulence as well — namely that they can cause cancer via insertional mutagenesis...

19. Antibodies 101: Producing Recombinant Antibodies

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    ...column or an ultrafiltration concentrator. As the name suggests, you may also concentrate your prep to ...

20. MXS Chaining

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    ...can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression, or a ...