We narrowed to 391 results for: NAM

1. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   ...pioneered by Voie and colleagues and originally named orthogonal-plane fluorescence optical sectioning...

2. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   ...manipulated to express more than two genes. Plasmid Name Multicistronic Element Expression Type MSCV-IRES-EGFP...

3. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ...result should match, enter the common or scientific name into the Organism box. This small piece of information...

4. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...Nanobody Plasmids Structural comparison As the name suggests, nanobodies are smaller antibodies.They...

5. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   ...teachers, who work hard maintaining a current, dynamic curriculum, would be hard-pressed to maintain the...

6. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ... your primers and template. Amplifying the ‘unamplifiable’ Check your amplification by gel electrophoresis...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many thanks ...

8. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   ...and identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing ...

9. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ...repository has played in these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth...

10. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...diluting the virus. It is important to note that the dynamic range of the ddPCR is between 1 and 100,000 genome...

11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    ...? The available filtering options now change dynamically based on the type of material and specific search...

12. MXS Chaining

    Type

    Blog Post

    ...can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression, or a ...

13. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    ...Cross-Linkers Chemical cross-linkers work by (as the name suggests) cross-linking cellular proteins. Cross-linking...

14. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    ...column or an ultrafiltration concentrator. As the name suggests, you may also concentrate your prep to ...

15. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...have their own unusual sort of virulence as well — namely that they can cause cancer via insertional mutagenesis...

16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    ...into bacterial genomes. This new capability was named INTEGRATE (Insertion of Transposable Elements by...

17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ... D. Lavis, and Eric Betzig. "Imaging live-cell dynamics and structure at the single-molecule level." Molecular...

18. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ...3D fractal). Enhancer biology is a complex and dynamic field. We’re going to focus on a tried and true...

19. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    ...potentially overcome some of Cas9’s shortcomings - namely its blunt double stranded cleavage and G-rich PAM...

20. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ...engineered DNA-binding molecules for the first time and named the new technology as engineered DNA-binding molecule-mediated...