We narrowed to 423 results for: NUC

1. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   ...presence of a viral protein, such as p24, or viral nucleic acid. Functional titers, or infectious titers, ...

2. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   ... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine...

3. Plasmids 101: Protein Expression

   Type

   Blog Post

   ... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   ...xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine ...

5. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   ...repair harmful double stranded breaks, in which nucleotide sequences are exchanged between two similar or...

6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   ... randomized with a defined set of different trinucleotides (corresponding to different amino acids) in...

7. Hot Plasmids: Winter 2025

   Type

   Blog Post

   ...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right...

8. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   ...ligase fidelity and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed...

9. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   ...traversing pons and medulla, bp= basilar pons, NST nucleus of the solitary tract.  The arrows point the direction...

10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ... from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. I spoke with her ...

11. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    ...imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol...

12. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol

    ...improves transduction efficiency by facilitating nuclear import of the vector’s preintegration complex in...found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine (A,G), and Y is a pyrimidine (...targeting introns. Avoid stretches of 4 or more nucleotide repeats, especially repeated Ts because polyT...program. Select sequences that have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene...

13. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    ...because they remain in episomal form in the cell’s nucleus. But since nothing’s ever perfect, neither is ...

14. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...African descent have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the...

15. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ...cell type, even within the same brain region or nucleus. Just optimizing AAV expression can take months...

16. Getting to Know Expansion Microscopy

    Type

    Blog Post

    ...with SRM techniques, and adapted for detecting nucleic acids as well as proteins (Klimas, et al. 2023;...

17. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ... cloning by homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID:...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ...fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging, not for live-cell...

19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ...think of DNA as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong....

20. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope...