We narrowed to 362 results for: Nov

1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   ...permitted researchers to longitudinally map the turnover of Langerhans cells in the dermis and identify...

2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   Blog Post

   ...mechanisms of Alzheimer’s disease by developing novel iPSC-based model systems.     References 1. Cong...

3. Plasmids 101: Biotinylation

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   Blog Post

   ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

4. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   ...research, and we’re looking forward to even more innovation next year! Did we miss any? Let us know about...

5. Antibodies 101: Introduction to Immunofluorescence

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   ...    References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...

6. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ...Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber...

7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   ...roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within...

8. Hot Plasmids: Fall 2025

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   ...proliferation of leukemia cells, and assembly of adenovirus particles (Zhang et al., 2025). Figure...

9. Exploring Applications of the Bioluminescent HiBiT Tag

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   ...luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chemical Biology...

10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4...

11. Generating Mouse Models Using CRISPR/Cas9

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    ...Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not ...

12. Viral Vectors 101: Optogenetic Tools

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    ...Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated chimeric GPCRs: Limitations...

13. Antibodies 101: Multiplex Immunofluorescence

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    ...doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An ...

14. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...silencing in vivo by programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427...

15. Antibodies 101: Antibody Engineering and Directed Evolution

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    ...engineering technologies, the Institute of Protein Innovation (IPI) provides information on their large-scale...

16. When Fidelity Matters: A frank discussion about ligase fidelity

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    ... of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary...

17. Transferable Skills Guide: Teamwork

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    Blog Post

    ...The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but even the movie makers saw how ...

18. Important Considerations When Using AAVs

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    ...Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune response...

19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin...

20. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...Andrew, and Jamie Maguire. "Characterization of a novel subtype of hippocampal interneurons that express...