We narrowed to 359 results for: Nov

1. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   ...research, and we’re looking forward to even more innovation next year! Did we miss any? Let us know about...

2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...permitted researchers to longitudinally map the turnover of Langerhans cells in the dermis and identify...

3. Plasmids 101: Biotinylation

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   ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

4. Antibodies 101: Introduction to Immunofluorescence

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   ...    References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...

5. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   ...Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber...

6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   ...roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within...

7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...mechanisms of Alzheimer’s disease by developing novel iPSC-based model systems.     References 1. Cong...

8. Viral Vectors 101: Optogenetic Tools

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   ...Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated chimeric GPCRs: Limitations...

9. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ...doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An ...

10. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...silencing in vivo by programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427...

11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4...

12. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ...Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not ...

13. Important Considerations When Using AAVs

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    ...Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune response...

14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin...

15. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ... of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary...

16. Transferable Skills Guide: Teamwork

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    Blog Post

    ...The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but even the movie makers saw how ...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform...

18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...doi.org/10.1021/ac2033112 Ligasová A, Vydržalová M, Buriánová R, Brůčková L, Večeřová R, Janošťáková A, Koberna...

19. Finding Your Science Policy Path

    Type

    Blog Post

    ...interested in driving scientific and technological innovation that is accessible and equitable for all. You...

20. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...Andrew, and Jamie Maguire. "Characterization of a novel subtype of hippocampal interneurons that express...