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We narrowed to 395 results for: POR C

Showing: 321 - 340 of 395 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ..., Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein...

  2. CRISPR Antimicrobials

    Type
    Blog Post
    ...reducing the chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help...colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just...

  3. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2 and ...recent lineage tracing approaches that led to important insights in developmental biology. Microscopy-...pathway regulated by one-eyed pinhead, incorrect proportions of otherwise normal cell types are produced, ...

  4. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail are injected...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...

  5. How to Write a Scientific Review Article

    Type
    Blog Post
    ...perfect. Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  As the name implies, this promoter... to therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

  7. DNA Purification Without a Kit

    Type
    Blog Post
    ...Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall, P.J., Trau, M., & Botella, J.R. (2017)...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...

  9. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. There are therefore...isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry... has developed several tools useful for the incorporation phosphoserine into your gene of interest. Depositor...University changed the way researchers can explore important questions surrounding serine phosphorylation by...in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP...able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...188531 ; 188532 ; 188533 ; 188534 ; 188535 ). Importantly, phosphorylation positional isomers are allocated...

  11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...either the PAM (changing one of the G’s to an A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence...it is incorporated. We were surprised to see how rapidly the efficiency of mutation incorporation falls...contains the intended sequence changes to be incorporated into the edited genome. In mammalian cells such...these two strategies allows you to specifically incorporate homo- or heterozygous mutations. 1. Increasing...therefore strongly encourage any researcher to incorporate CRISPR/Cas-blocking mutations at both targeted... our frustration) in many cases we only saw incorporation of the blocking mutation. Despite the fact that... away from the cut site were not efficiently incorporated during HDR. We decided to systematically characterize...

  12. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...PubMed PMID: 7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed...genes important for mesoderm differentiation Now, let's turn tinman and bagpipe, two genes important for...protein Groucho, a transcriptional co-repressor important in development. Groucho family proteins are found...Dana Farber Cancer Institute! Sonic Hedgehog, an important regulator of embryonic development The largest...video game character but rather to an extremely important gene in animal development. To understand where... Tinman, 2016). Bagpipe on the other hand, is important in the formation of the visceral mesoderm which...

  13. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...habitats - even 50 meters from Old Faithful in 60 °C soils. Dendrobatidae (Lauren O’Connell)  Poison frogs...number of systems that can be studied by providing support "for development of tools, approaches, and infrastructure...great for studying parenting strategies and make important ecological indicators since species live in water...Aiptasia - algae Breviolum minutum symbionts are important models for solutions to the coral reef crisis.... interesting biologically. They have economic importance as harmful weeds. The parasitic fusion is such...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...2.50021-0  Toh, W. K., Teo, Y. L., Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive...methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus, Neisseria spp., Paracoccous...Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp...Xanthomonas campestris   In addition to incorporating broad host range replicons, recombinant vectors...can be expressed. For example, scientists can incorporate interchangeable antibiotic resistance cassettes...

  15. Genetic Code Expansion

    Type
    Collection
    ...synthetase subunit E. coli p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA...(CePheRS) C.elegans p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA... the codon), the amino acid from the tRNA is incorporated into the growing peptide chain. To expand the...aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid ...the cells, the non-canonical amino acid can be incorporated using the existing protein translation machinery...translation machinery are needed in order to incorporate a non-canonical amino acid into the protein of... the place where the new amino acid would be incorporated. Other options, such a 4-base pair codons, have...

  16. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Barlow-Anacker, A., Bochkov, Y., Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World...

  17. CRISPR References and Information

    Type
    Collection
    ...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans )...gRNA and HDR donor design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes...Lab . Read more about it on the Addgene blog . CRISPOR (Link opens in a new window) This tool helps design...(2016) (Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral...input sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms... or a sequence text file you upload. Currently supports a wide selection of organisms. From the Database...sequence and checks for off-target binding. Currently supports a wide range of organims. Developed by the Michael...

  19. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... internal tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events..., D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions...FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in... a cell and depends on the efficiency of BV incorporation by an apoprotein and protein expression level...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...

  20. Evolution of Lab Techniques

    Type
    Blog Post
    ...1975): 503-517. PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test...although they are reported to have high error rates (10). It is projected that nanopore-based sequencing... similar experimental questions (see below). Importantly, although their role has changed, restriction...lengths because extension is terminated upon the incorporation of a ddNTP. Researchers perform four different...activity as fluorescently labeled nucleotides are incorporated, a method called pyrosequencing. The drive for...sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads the nucleotides...nucleotides of a DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the...
Showing: 321 - 340 of 395 results