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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 663 results for: PPL;

Showing: 321 - 340 of 663 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3.   Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2.   Improved trafficking and localization...

  3. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... have profound impacts on the development of applications with the potential to save lives. While most...daunting because they have no clear cut answers, I applaud the stem cell community for addressing these important...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...

  5. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications.   References Refactoring and optimization ...

  6. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...

  8. Clear and Accessible Writing

    Type
    Blog Post
    ...principles we used in our daily content writing applied to alt text. We also were aware of the need for...start small with alt text or immediately dive into applying these four steps elsewhere, engaging in this practice...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  11. Antibodies 101: ChIP

    Type
    Blog Post
    ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...) How many loci can Cas9 modify? To test the application of this system to gRNA multiplexing, Xie et al...multiplexing further illustrates the potential of PTGs. Applications of PTGs The major advantage of the PTG system...

  16. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...associated with molecular plant parts, particularly for applied projects intended for commercialization. Many of...patented, and building our own novel pieces where applicable. We will also be working with open source, transformation...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...
Showing: 321 - 340 of 663 results