We narrowed to 452 results for: SUP

1. Finding and Joining Your Dream Lab

   Type

   Blog Post

   ...considering this, make sure to choose an advisor that supports both paths – you won't know in year 1 what you...

2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   ...nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from...

3. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   ... stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP...

4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   .... J. R. Huh et al. Digoxin and its derivatives suppress TH17 cell differentiation by antagonizing RORγt...

5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

6. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   ...learning how to train a dog with my daughter. It’s super fun and we are enjoying that immensely. Paratore...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ... above (envelope, packaging, and transfer) are supplied by three types of plasmids, which are cotransfected...

8. AAV Q&A with Tim Miles

   Type

   Blog Post

   ... lot of effort in immunomodulation or immune suppression to allow AAV re-administration but I do not think...

9. Antibodies 101: ChIP

   Type

   Blog Post

   ... debris, which will collect in a pellet. The supernatant will contain your fragmented, crosslinked chromatin...

10. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    ...fluorophores vary in brightness. You don’t want a super-bright fluorophore, like PE, on an abundant target...

11. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    ...can also allow multiplexing with experiments that supply both substrates and measure luciferase output at...

12. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

13. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    ...lentivirus are now available from Addgene. While super useful, these vectors require a bit of expertise...

14. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    ...certain media components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may ...

15. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    ...build the plant synthetic biology community and to support more efficient engineering of a better tomorrow...

16. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    ...DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...

17. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    ...’s affected by climate change or affected by a superbug. I think we solve these problems through connections...

18. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    ...he mean it the same way as Bryan, our customer support manager? When Gizela, the aforementioned lab manager...

19. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    ... because in vitro conditions did not optimally support growth of diverse species. These results further...

20. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...almost any career. It allows you to share and build support for your ideas, invite others into decision making...