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We narrowed to 749 results for: UTY

Showing: 321 - 340 of 749 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to engineer two new IV ...stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant AAV capsid...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...

  2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ... GCaMP containing plasmids are available for distribution. If your experiment involves delivering a calcium...types of searches including depositing labs and institutions. For example, search for “Melina Fan” and you...Lab published in eLife. Addgene is currently distributing 94 plasmids expressing validated antibodies ...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...They then performed sixteen rounds of directed evolution across the full ADAR2DD to further optimize cytidine...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    ... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses...KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and elution steps use very moderate buffer conditions FLAG...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...by Gaudelli et al. resulted in the successful evolution of an adenosine deaminase that can act on DNA ...for surviving colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA...in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 hours...line was obtained directly from Takara, and is routinely tested for mycoplasma contamination using mycoplasma...Cells transduced with our lentiviral vectors are routinely checked for signs of microbial contamination. ...

  7. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... Variation in ori structure allows plasmids to utilize diverse host replication machinery. For instance...Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae... P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ... variety of fields in order to provide useful solutions to complex challenges. As a cross-disciplinary...citation rates to measure the impact of their contribution. It offers a 5-step process and several examples...Social Networking for Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion...

  9. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...; multiple codons encode single amino acids. Evolutionary constraints have molded which codons are used...express well in any organism. So what are the evolutionary constraints that lead to these preferences and...6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation of codon optimality reveals hidden...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...collaborators of Omar and Jonathan and experts in CRISPR evolution, were also working on the project. Using computational...possibilities at the bench and potentially in therapeutic applications. The potential of CRISPR has yet...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al, 2020). Because retrovirus is ...disadvantages: Table 1 (Joglekar et al., 2017)  Table 1 (Gutierrez-Guerrero et al., 2020) Table 1 (Cronin et al.,...–222 . https://doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral...

  12. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

  13. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...resistance marker from the vector (abR, Figure 1). The resolution step (“double crossover”) which leads to the ... program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  14. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...fluorescent aptamer, was identified through directed evolution. First, a pool of RNAs that activate the fluorescence...fluorescence of DFHBI were identified using systematic evolution of ligands by exponential enrichment (SELEX). ... by fluorescence-based selection and directed evolution." Journal of the American Chemical Society 136.46...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine...matter. Plus, for folks interested in directed-evolution methods, the activity-based selection in mammalian...Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells. Nature...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce... bases at the 3’-end interfere with plasmid reconstitution, and consequently Taq polymerases cannot be...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...external forces such as ionizing radiation, chemotherapeutics, and ultraviolet light. In this post, we will...via cleavage in one of two ways - each junction resolution could happen on the crossing strand (horizontally...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...

  18. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...be dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...much to learn about their myriad functions and therapeutic potential. Our partners at the Institute for ...proteins. Lucky for us, we now have a two-for-one solution: TurboCas!TurboCas provides the genomic targeting...-type-specific expression has been a promising solution. Recently, the labs of Gord Fishell and colleagues...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! This post was originally published...efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes. Scientific Reports, 15(... AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic...
Showing: 321 - 340 of 749 results