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We narrowed to 770 results for: VARS

Showing: 321 - 340 of 770 results

  1. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...Institute today, where he helps researchers in a variety of fields develop RNAi screens to answer their ...more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the ...Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate the...

  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...and writer.” Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor...

  3. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...throughout the year since hiring opportunities arise at various times. If the timing and interests align for both...

  5. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...pressure to select for variants of interest, though. A selection can take the form of varied temperature, nutrient...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    ...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

  7. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion...thoroughly validated. The creativity in employing a variety of cloning techniques and troubleshooting methodologies...

  8. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...to reduce the chances of making an error. Try a variety of things, including (and maybe especially) suggestions...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

  11. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...editing in both bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein...efficiency of prime editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology... Various Addgenies...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

  14. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...this is the case, you can narrow them down in a variety of ways. First, review the citations. An antibody...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction detection...

  16. To Each HIS Own

    Type
    Blog Post
    ...tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity...acid sequence, or tag, on the protein. While a variety of methods can be used to successfully isolate ...tools for studying proteins and are used for a variety of assays in the lab (for a refresher see Antibodies...

  17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... is explained in more detail here. There are a variety of methods that can be used to measure your fluorescent...filtration (Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay ... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

  18. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...different fluorescence imaging strategies use widely varying amounts of excitation power (Shaner et al, 2008... An example of a photostability measurement of various cyan fluorescent proteins is depicted in figure...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

  19. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...lab researcher Jonathan Tang wanted to express a variety of proteins in single cell types in the mouse, ...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...
Showing: 321 - 340 of 770 results