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We narrowed to 573 results for: Vit

Showing: 321 - 340 of 573 results

  1. DNA Purification Without a Kit

    Type
    Blog Post
    ... but in less time. The authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours... faster, cheaper, and had a similar level of sensitivity. Figure 2: Steps to purify DNA using a ...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...figures 4A-C). And since they have limited cross-reactivity between different IgG subclasses and different...State University Use Nanobodies to Control Protein Activity Learn more about protein tags Read about E. coli...

  3. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env,... particles with the VSV-G envelope have high infectivity in a wide range of cell types, so they’re ideal...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...expressed as fusions that retain their individual activities (1). Researchers also discovered the modular ...activation (TA) domains retain their individual activities when separated (2), and that DBD and TAs from...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord,  once you have these rules...

  10. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision...

  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ..., M., & Al-Qahtani, M. (2018). Evaluation of in vitro chondrocytic differentiation: A stem cell research...Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T ...

  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...fusing mutants of Cas9 deficient in DNA cutting activity to a transcriptional activation domain (Fig 1)...Worry less about off targets Unlike Cas9 cutting activity, off target effects are generally not regarded...

  16. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...using microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within...used to measure conformational changes, cleavage activity using FRET-based biosensors, and interactions ...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...indirect labeling is through antibody host species reactivity. Just like for western blotting, a primary epitope-targeting...biotin-streptavidin binding and antibody host species reactivity. When sorting cells with a high resolution for...
Showing: 321 - 340 of 573 results